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Literature summary for 1.15.1.1 extracted from

  • Amo, T.; Atomi, H.; Imanaka, T.
    Biochemical properties and regulated gene expression of the superoxide dismutase from the facultatively aerobic hyperthermophile Pyrobaculum calidifontis (2003), J. Bacteriol., 185, 6340-6347.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli. Although the recombinant protein is soluble, little activity is observed due to the lack of metal incorporation. Reconstitution of the enzyme by heat treatment with either Mn2+ or Fe2+ yields a highly active protein Pyrobaculum calidifontis

Inhibitors

Inhibitors Comment Organism Structure
azide Mn2+-reconstituted recombinant enzyme and Fe2+-reconstututed recombinant enzyme displays relatively strong resistance against azide. Mn2+- and Fe2+-reconstituted activity decreases 50% with 380 and 340 mM azide, respectively Pyrobaculum calidifontis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Pyrobaculum calidifontis 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ native enzyme from aerobically-grown cells grown in standard medium contains 0.55 mol Fe2+ per mol of subunit. Native enzyme from aerobically-grown cells grown in medium supplemented with manganese contains less than 0.01 mol Fe2+ per mol of subunit. Native enzyme from aerobically-grown cells grown in medium supplemented with iron contains 0.01 mol Fe2+ per mol of subunit. Native enzyme from anaerobically-grown cells grown in standard medium contains 0.43 mol Fe2+ per mol of subunit. Recombinant apo-enzyme contains less than 0.01 mol Fe2+ per mol of subunit. Mn2+-reconstituted recombinant enzyme contains less than 0.01 mol Fe2+ per mol of subunit. Fe2+-reconstituted recombinant enzyme contains 0.76 mol Mn2+ per mol of subunit. The recombinant protein has little activity due to the lack of metal incorporation. Reconstitution of the enzyme by heat treatment with either Mn2+ or Fe2+ yields a highly active protein Pyrobaculum calidifontis
Mn2+ native enzyme from aerobically-grown cells grown in standard medium contains 0.55 mol Mn2+ per mol of subunit. Native enzyme from aerobically-grown cells grown in medium supplemented with manganese contains 0.86 mol Mn2+ per mol of subunit. Native enzyme from aerobically-grown cells grown in medium supplemented with iron contains 0.68 mol Mn2+ per mol of subunit. Native enzyme from anaerobically-grown cells grown in standard medium contains 0.08 mol Mn2+ per mol of subunit. Recombinant apoenzyme contains less than 0.01 mol Mn2+ per m,ol of subunit. Mn2+-reconstituted recombinant enzyme contains 0.86 mol Mn2+ per mol of subunit. Fe2+-reconstituted recombinant enzyme contains less than 0.01 mol Mn2+ per mol of subunit.The recombinant protein has little activity due to the lack of metal incorporation. Reconstitution of the enzyme by heat treatment with either Mn2+ or Fe2+ yields a highly active protein Pyrobaculum calidifontis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
x * 24000, SDS-PAGE Pyrobaculum calidifontis
61000
-
gel filtration Pyrobaculum calidifontis

Organism

Organism UniProt Comment Textmining
Pyrobaculum calidifontis Q76CY6
-
-
Pyrobaculum calidifontis JCM 11548 Q76CY6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrobaculum calidifontis

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:aerobically-grown cell
-
Pyrobaculum calidifontis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
434
-
recombinant Fe2+-reconstituted enzyme, pH and temperature not specified in the publication Pyrobaculum calidifontis
1960
-
purified native enzyme, pH and temperature not specified in the publication Pyrobaculum calidifontis
1970
-
recombinant Mn2+-reconstituted enzyme, pH and temperature not specified in the publication Pyrobaculum calidifontis

Subunits

Subunits Comment Organism
? x * 24000, SDS-PAGE Pyrobaculum calidifontis

Synonyms

Synonyms Comment Organism
SOD
-
Pyrobaculum calidifontis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
the Mn2+-reconstituted recombinant enzyme is not inactivated at all after 5 h of incubation at 100°C. A 1 h incubation leads to a 50% decrease in the activity of the Fe2+-reconstituted enzyme Pyrobaculum calidifontis