Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli. Although the recombinant protein is soluble, little activity is observed due to the lack of metal incorporation. Reconstitution of the enzyme by heat treatment with either Mn2+ or Fe2+ yields a highly active protein | Pyrobaculum calidifontis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | Mn2+-reconstituted recombinant enzyme and Fe2+-reconstututed recombinant enzyme displays relatively strong resistance against azide. Mn2+- and Fe2+-reconstituted activity decreases 50% with 380 and 340 mM azide, respectively | Pyrobaculum calidifontis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Pyrobaculum calidifontis | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | native enzyme from aerobically-grown cells grown in standard medium contains 0.55 mol Fe2+ per mol of subunit. Native enzyme from aerobically-grown cells grown in medium supplemented with manganese contains less than 0.01 mol Fe2+ per mol of subunit. Native enzyme from aerobically-grown cells grown in medium supplemented with iron contains 0.01 mol Fe2+ per mol of subunit. Native enzyme from anaerobically-grown cells grown in standard medium contains 0.43 mol Fe2+ per mol of subunit. Recombinant apo-enzyme contains less than 0.01 mol Fe2+ per mol of subunit. Mn2+-reconstituted recombinant enzyme contains less than 0.01 mol Fe2+ per mol of subunit. Fe2+-reconstituted recombinant enzyme contains 0.76 mol Mn2+ per mol of subunit. The recombinant protein has little activity due to the lack of metal incorporation. Reconstitution of the enzyme by heat treatment with either Mn2+ or Fe2+ yields a highly active protein | Pyrobaculum calidifontis | |
Mn2+ | native enzyme from aerobically-grown cells grown in standard medium contains 0.55 mol Mn2+ per mol of subunit. Native enzyme from aerobically-grown cells grown in medium supplemented with manganese contains 0.86 mol Mn2+ per mol of subunit. Native enzyme from aerobically-grown cells grown in medium supplemented with iron contains 0.68 mol Mn2+ per mol of subunit. Native enzyme from anaerobically-grown cells grown in standard medium contains 0.08 mol Mn2+ per mol of subunit. Recombinant apoenzyme contains less than 0.01 mol Mn2+ per m,ol of subunit. Mn2+-reconstituted recombinant enzyme contains 0.86 mol Mn2+ per mol of subunit. Fe2+-reconstituted recombinant enzyme contains less than 0.01 mol Mn2+ per mol of subunit.The recombinant protein has little activity due to the lack of metal incorporation. Reconstitution of the enzyme by heat treatment with either Mn2+ or Fe2+ yields a highly active protein | Pyrobaculum calidifontis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
x * 24000, SDS-PAGE | Pyrobaculum calidifontis |
61000 | - |
gel filtration | Pyrobaculum calidifontis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrobaculum calidifontis | Q76CY6 | - |
- |
Pyrobaculum calidifontis JCM 11548 | Q76CY6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrobaculum calidifontis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:aerobically-grown cell | - |
Pyrobaculum calidifontis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
434 | - |
recombinant Fe2+-reconstituted enzyme, pH and temperature not specified in the publication | Pyrobaculum calidifontis |
1960 | - |
purified native enzyme, pH and temperature not specified in the publication | Pyrobaculum calidifontis |
1970 | - |
recombinant Mn2+-reconstituted enzyme, pH and temperature not specified in the publication | Pyrobaculum calidifontis |
Subunits | Comment | Organism |
---|---|---|
? | x * 24000, SDS-PAGE | Pyrobaculum calidifontis |
Synonyms | Comment | Organism |
---|---|---|
SOD | - |
Pyrobaculum calidifontis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
the Mn2+-reconstituted recombinant enzyme is not inactivated at all after 5 h of incubation at 100°C. A 1 h incubation leads to a 50% decrease in the activity of the Fe2+-reconstituted enzyme | Pyrobaculum calidifontis |