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Literature summary for 1.15.1.1 extracted from

  • Nonaka, K.; Yoon, K.S.; Ogo, S.
    Biochemical characterization of psychrophilic Mn-superoxide dismutase from newly isolated Exiguobacterium sp. OS-77 (2014), Extremophiles, 18, 363-373.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene sodA, DNA and amino acid sequence determination and analysis, sequence comparison Exiguobacterium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ MnSOD, the Mn ion is the only metal cofactor, 0.57 atom per polypeptide chain Exiguobacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22532
-
2 * 23500, SDS-PAGE, 2 * 22532, sequence calculation Exiguobacterium sp.
23500
-
2 * 23500, SDS-PAGE, 2 * 22532, sequence calculation Exiguobacterium sp.
50000
-
gel filtration Exiguobacterium sp.

Organism

Organism UniProt Comment Textmining
Exiguobacterium sp.
-
collected from a tepid spring, 41°C, pH 7.9, in Oguni-tyo, Kumamoto, Japan, gene sodA
-
Exiguobacterium sp. OS-77
-
collected from a tepid spring, 41°C, pH 7.9, in Oguni-tyo, Kumamoto, Japan, gene sodA
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 50fold from strain OS-77 by hydrophobic interaction and anion exchange chromatography, followed by gel filtration Exiguobacterium sp.

Source Tissue

Source Tissue Comment Organism Textmining
additional information growth temperature range of the strain OS-77 is between 5°C and 40°C with its optimum growth temperature of 20°C, the growth pH range of the strain OS-77 is between pH 6.0 and pH 10.0 with optimum growth at pH 8.0, strain OS-77 shows salinity tolerance up to 7.5% w/v NaCl solution Exiguobacterium sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10500
-
purified native enzyme, pH 7.8, 25°C Exiguobacterium sp.

Subunits

Subunits Comment Organism
homodimer 2 * 23500, SDS-PAGE, 2 * 22532, sequence calculation Exiguobacterium sp.

Synonyms

Synonyms Comment Organism
MnSOD
-
Exiguobacterium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5
-
-
Exiguobacterium sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 25 optimum activity at 5°C, 80% of maximal activity at 25°C Exiguobacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
two protein denaturation peaks at 65°C and 84°C by differential scanning calorimetry Exiguobacterium sp.
45
-
stable up to Exiguobacterium sp.
60
-
21.2 min, loss of 50% activity Exiguobacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Exiguobacterium sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 11 70% of maximal activity within this range Exiguobacterium sp.

General Information

General Information Comment Organism
additional information residues involved in the active site are H28, Y36, H82, Q149, D164, and H168, which are completely conserved in the EgMnSOD Exiguobacterium sp.
physiological function the enzyme may play an important role as an antioxidant in a wide temperature range Exiguobacterium sp.