Cloned (Comment) | Organism |
---|---|
gene CuZnSOD, DNA and amino acid sequence determination and analysis, recombinant His6-tagged Cp-icCuZnSOD with high enzyme activity is induced to be expressed in Escherichia coli strain BL21(DE3) as a soluble form by IPTG supplemented with Cu/Zn ions at 20°C for 8 h | Cristaria plicata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
SDS | recombinant Cp-icCuZnSOD is active and retains more than 80% activity under treatment with 1-6% SDS. It retains 70% activity after treatment with 8% SDS but activity is rapidly lowered to 52% after treatment with 10% SDS | Cristaria plicata |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | Cp-icCuZnSOD contains no signal peptide | Cristaria plicata | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | an intracellular Cu-Zn superoxide dismutase. The enzyme amino acid sequence contains several highly conserved motifs including Cu/Zn ions binding sites, i.e. His46, His48, His63, and His120 for Cu2+ binding | Cristaria plicata | |
Zn2+ | an intracellular Cu-Zn superoxide dismutase. The enzyme amino acid sequence contains several highly conserved motifs including Cu/Zn ions binding sites, i.e. His63, His71, His80, and Asp83 for Zn2+ binding | Cristaria plicata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cristaria plicata | - |
gene CuZnSOD | - |
Purification (Comment) | Organism |
---|---|
recombinant soluble Cp-icCuZnSOD by nickel affinity chromatography | Cristaria plicata |
Synonyms | Comment | Organism |
---|---|---|
CpSOD | - |
Cristaria plicata |
CuZnSOD | - |
Cristaria plicata |
intracellular Cu-Zn superoxide dismutase | - |
Cristaria plicata |
More | the enzyme belongs to the superoxide dismutase family of important antioxidant metalloenzymes that catalyze superoxide radicals from cellular oxidative metabolism into hydrogen peroxideand molecular oxygen | Cristaria plicata |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 90 | the recombinant enzyme retains more than 80% activity between 10°C and 60°C, but loses activity rapidly, which is reduced to 54% and 40% at 70°C and 80°C, respectively, and it is almost inactive at 90°C | Cristaria plicata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Cristaria plicata |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 9 | activity range, the enzyme shows 30% of maximal activity at pH 10.0 and is inactive at pH 11.0 | Cristaria plicata |
General Information | Comment | Organism |
---|---|---|
additional information | CpSOD contains an intracellular disulfide bond and two CuZnSOD family signatures | Cristaria plicata |
physiological function | protection by recombinant Cp-icCuZnSOD against alcohol-injury in human hepatocyte L02 cell line | Cristaria plicata |