Cloned (Comment) | Organism |
---|---|
gene sodB, overexpression of the His6-tagged type A isozyme in Escherichia coli strain BL21(DE3) | Helicobacter pylori |
Crystallization (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme, hanging drop vapor diffusion method, 10 mg/ml protein in 20 mM Tris-HCl, pH 8.0, at 23°C, mixing of 0.001 ml protein solution with 0.001 ml precipitant solution containing 1.9 M ammonium sulfate in 0.2 M Tris-HCl buffer, pH 8.0, X-ray diffraction structure determination and analysis at 2.4 A resolution, modelling | Helicobacter pylori |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | Fe-SOD contains 1 Fe2+ per subunit | Helicobacter pylori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
O2.- + H+ | Helicobacter pylori | a key enzyme for fighting oxidative stress | O2 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | - |
contains a single type A isozyme Fe-SOD encoded by gene sodB | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged type A isozyme from Escherichia coli strain BL21(DE3) by His-trap affinity chromatography and gel filtration | Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O2.- + H+ | - |
Helicobacter pylori | O2 + H2O2 | - |
? | |
O2.- + H+ | a key enzyme for fighting oxidative stress | Helicobacter pylori | O2 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | the homodimer contains an extended C-terminal tail comprising residues 193-213. Dimer interface and domain interface structure analysis: the shortest domain contacts involve residues Phe17, Leu52, Phe53, Tyr71, and Phe75 on one domain and Val145, Pro151, Val154, Tyr173, Phe177, His180, Cys189, Leu198, Ile205, and His210 on the other domain, and the dimer interface is stabilized by 10 hydrogen bonds and approximately 102 non-bonded contacts, involving 32 residues from both chains, overview | Helicobacter pylori |
Synonyms | Comment | Organism |
---|---|---|
SOD | - |
Helicobacter pylori |