Crystallization (Comment) | Organism |
---|---|
crystal structures of unfluorinated and fluorinated enzyme are nearly superimposable. Ratio kcat/Km decreases from 0.8 per mM and s for wild-type to 0.03 per mM and s for the fluorinated mutant which is in significant part due to 3-fluorotyrosine residues distant from the active-site metal | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | replacement of all of the nine tyosine residues in each of the four enzyme subunits by 3-fluorotyrosine. Crystal structures of unfluorinated and fluorinated enzyme are nearly superimposable. Ratio kcat/Km decreases from 0.8 per mM and s for wild-type to 0.03 per mM and s for the fluorinated mutant which is in significant part due to 3-fluorotyrosine residues distant from the active-site metal | Homo sapiens |
Y34F | about 12fold decrease in kcat value | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
manganese superoxide dismutase | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O2- + H+ | - |
Homo sapiens | O2 + H2O2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1100 | - |
O2- | pH 7.5, 25°C, mutant Y34F with fluorinated Tyr-residues | Homo sapiens | |
3300 | - |
O2- | pH 7.5, 25°C, mutant Y34F | Homo sapiens | |
4600 | - |
O2- | pH 7.5, 25°C, wild-type with fluorinated Tyr-residues | Homo sapiens | |
40000 | - |
O2- | pH 7.5, 25°C, wild-type | Homo sapiens |