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Literature summary for 1.15.1.1 extracted from

  • Sato, S.; Harris, J.I.
    Superoxide dismutase from Thermus aquaticus. Isolation and characterisation of manganese and apo enzymes (1977), Eur. J. Biochem., 73, 373-381.
    View publication on PubMed
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General Stability

General Stability Organism
guanidinium chloride: 6 M, pH 7.5, 16 h, 23°C, stable Thermus aquaticus
urea: 8 M, pH 7.5, 16 h, 23°C, stable Thermus aquaticus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 2 atoms of manganese per molecule Thermus aquaticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
 4 * 21000, Mn-SOD, SDS-PAGE Thermus aquaticus
80000
-
 Mn-SOD, gel filtration Thermus aquaticus

Organism

Organism UniProt Comment Textmining
Thermus aquaticus
-
 Mn-SOD
-
Thermus aquaticus Mn-SOD
-
 Mn-SOD
-

Purification (Commentary)

Purification (Comment) Organism
Mn-SOD Thermus aquaticus

Renatured (Commentary)

Renatured (Comment) Organism
reconstitution of active enzyme after withdrawal of Mn2+ by addition of Mn2+ to apoprotein in 8 M urea at acid pH Thermus aquaticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Thermus aquaticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O2- + H+
-
 Thermus aquaticus O2 + H2O2
-
 ?
O2- + H+
-
 Thermus aquaticus Mn-SOD O2 + H2O2
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 21000, Mn-SOD, SDS-PAGE Thermus aquaticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
 10 min, stable up to Thermus aquaticus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10.5 12 h, 23°C, stable Thermus aquaticus