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Literature summary for 1.14.99.66 extracted from

  • Lee, M.G.; Wynder, C.; Bochar, D.A.; Hakimi, M.A.; Cooch, N.; Shiekhattar, R.
    Functional interplay between histone demethylase and deacetylase enzymes (2006), Mol. Cell. Biol., 26, 6395-6402 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.99.66

Cloned(Commentary)

Cloned (Comment) Organism
gene KDM1A, recombinant expression of FLAG-tagged wild-type and mutant enzymes in Escherichia coli strain BL21, recombinant expression of FLAG-tagged wild-type enzyme in Spodoptera frugiperda SF21 cells via baculovrial expression system. Ectopic expression of the LSD1 enzymatically dead mutant K661A Homo sapiens

Protein Variants

Protein Variants Comment Organism
K661A enzymatically dead mutant of BHC110 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information histone deacetylase (HDAC) inhibitors are a promising class of anticancer agents for the treatment of solid and hematological malignancies. HDAC inhibitors diminish histone H3 lysine 4 (H3K4) demethylation by LSD1 in vitro. In vivo analysis reveals an increased H3K4 methylation concomitant with inhibition of nucleosomal deacetylation by HDAC inhibitors. Histone H3K4 demethylation is a secondary target of HDAC inhibitors Homo sapiens
sodium butyrate a histone deacetylase (HDAC) inhibitor Homo sapiens
trichostatin A a histone deacetylase (HDAC) inhibitor Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleosome
-
 Homo sapiens 786
-
nucleus
-
 Homo sapiens 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens functional interplay between histone demethylase and histone deacetylase ?
-
 ?
[histone H3]-N6,N6-dimethyl-L-lysine 4 + 2-oxoglutarate + O2 Homo sapiens
-
 [histone H3]-N6-methyl-L-lysine 4 + succinate + formaldehyde + CO2
-
 ?
[histone H3]-N6-methyl-L-lysine 4 + 2-oxoglutarate + O2 Homo sapiens
-
 [histone H3]-L-lysine 4 + succinate + formaldehyde + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O60341
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant FLAG-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 by M2 affinity chromatography, recombinant FLAG-tagged wild-type enzyme from insect Sf21 cells by M2 affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information functional interplay between histone demethylase and histone deacetylase Homo sapiens ?
-
 ?
additional information the bifunctional enzyme catalyzes the demethylation of H3K4me2/me1 and H3K9me2/me1 (EC 1.14.11.65). Functional interplay between histone demethylase and histone deacetylase. The enzymatic activities of the histone demethylase and the deacetylase are intimately linked. The cross talk between the two enzymes is seen only when nucleosomal substrates are used and is mediated through different domains of the CoREST protein (FLAG-tagged CoREST and mutants are recombinantly expressed in HEK-293 cells and Escherichia coli strain BL21). LSD1-HDAC1 complexes display enhanced acetylation activity in presence of CoREST Homo sapiens ?
-
 ?
[histone H3]-N6,N6-dimethyl-L-lysine 4 + 2-oxoglutarate + O2
-
 Homo sapiens [histone H3]-N6-methyl-L-lysine 4 + succinate + formaldehyde + CO2
-
 ?
[histone H3]-N6-methyl-L-lysine 4 + 2-oxoglutarate + O2
-
 Homo sapiens [histone H3]-L-lysine 4 + succinate + formaldehyde + CO2
-
 ?

Synonyms

Synonyms Comment Organism
BHC110
-
 Homo sapiens
KDM1A
-
 Homo sapiens
LSD1
-
 Homo sapiens
lysine-specific histone demethylase 1A UniProt Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD dependent on Homo sapiens

General Information

General Information Comment Organism
evolution histone methylation can be reversed by several histone demethylases, including PAD4/PADI4, BHC110/LSD1, and JmjC domain-containing demethylases. a family of multiprotein complexes contains histone deacetylase 1/2 (HDAC1/2) and the histone demethylase LSD1 (BHC110). LSD1 demethylates mono- and dimethyl histone H3 lysine 4 (H3K4) and belongs to a family of FAD-dependent polyamine oxidases which use molecular oxygen as an electron acceptor to oxidize an amine group Homo sapiens
metabolism a family of multiprotein complexes contains histone deacetylase 1/2 (HDAC1/2) and the histone demethylase BHC110 (LSD1). Reconstitution of recombinant complexes reveals a functional connection between HDAC1 and BHC110 only when nucleosomal substrates are present. The enzymatic activity of BHC110 is required to achieve optimal deacetylation in vitro. In vivo functional cross talk between the demethylase and deacetylase enzymes is detected. Demethylation of nucleosomes enhances deacetylation Homo sapiens