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Literature summary for 1.14.99.66 extracted from

  • Karytinos, A.; Forneris, F.; Profumo, A.; Ciossani, G.; Battaglioli, E.; Binda, C.; Mattevi, A.
    A novel mammalian flavin-dependent histone demethylase (2009), J. Biol. Chem., 284, 17775-17782.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.99.66

Cloned(Commentary)

Cloned (Comment) Organism
LSD2, DNA and amino acid sequence determination and analysis, expression of the His6-tagged wild-type enzyme and two truncated mutants in Escherichia coli strain BL21(DE3) Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information construction of two N-terminally truncated mutants, DELTA25 and DELTA257 Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
[histone H3 peptide 21mer]-L-arginine4 competitive inhibition of LSD2 Mus musculus
[histone H3 peptide 21mer]-L-glutamine4 competitive inhibition of LSD2 Mus musculus
[histone H3 peptide 21mer]-L-lysine4 the demethylated peptide, product of the LSD2 reaction, inhibits LSD2 Mus musculus
[histone H3 peptide 21mer]-L-methionine4 competitive inhibition of LSD2 Mus musculus
[histone H3 peptide 21mer]-N6,N6,N6-trimethyl-L-lysine4
-
 Mus musculus
[histone H3 peptide]-N6-methyl-L-lysine9
-
 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0051
-
 [histone H3 peptide 30mer]-N6-methyl-L-lysine4 pH 8.0, 25°C, recombinant wild-type LSD2 Mus musculus
0.0066
-
 [histone H3 peptide 21mer]-N6,N6-dimethyl-L-lysine4-dimethyl-L-lysine9 pH 8.0, 25°C, recombinant wild-type LSD2 Mus musculus
0.0081
-
 [histone H3 peptide 21mer]-N6-methyl-L-lysine4-methyl-L-arginine17 pH 8.0, 25°C, recombinant wild-type LSD2 Mus musculus
0.009
-
 [histone H3 peptide 21mer]-N6-methyl-L-lysine4-methyl-L-lysine9 pH 8.0, 25°C, recombinant wild-type LSD2 Mus musculus
0.0113
-
 [histone H3 peptide 21mer]-N6-methyl-L-lysine4 pH 8.0, 25°C, recombinant wild-type LSD2 Mus musculus
0.0125
-
 [histone H3 peptide 21mer P16A]-N6-methyl-L-lysine4 pH 8.0, 25°C, recombinant wild-type LSD2 Mus musculus
0.0705
-
 [histone H3 peptide 21mer]-N6-methyl-L-lysine4-acetyl-L-lysine9 pH 8.0, 25°C, recombinant wild-type LSD2 Mus musculus
0.092
-
 [histone H3 peptide 21mer]-N6-methyl-L-lysine4 pH 8.0, 25°C, recombinant wild-type LSD2 Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
 gene AOF1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged wild-type LSD2 and truncated mutants from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, followed by ultra- and gel filtration Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity and effect of epigenetic marks, overview. No activity with peptide substrate of chain length below 21. Activity determinations of recombinant wild-type and mutant enzymes by horseradish peroxidase-coupled and formaldehyde dehydrogenase-coupled assays with histone H3 peptides as substrates, method optimization, e.g. with respect to pH and ionic strength, overview Mus musculus ?
-
 ?
[histone H3 peptide 21mer P16A]-N6-methyl-L-lysine4 + 2-oxoglutarate + O2
-
 Mus musculus ?
-
 ?
[histone H3 peptide 21mer]-N6,N6-dimethyl-L-lysine4 + 2-oxoglutarate + O2
-
 Mus musculus [histone H3 peptide 21mer]-L-lysine4 + succinate + formaldehyde + CO2
-
 ?
[histone H3 peptide 21mer]-N6,N6-dimethyl-L-lysine4-dimethyl-L-lysine9 + 2-oxoglutarate + O2
-
 Mus musculus ?
-
 ?
[histone H3 peptide 21mer]-N6-methyl-L-lysine4 + 2-oxoglutarate + O2 no activity with a monomethylated H3-Lys4 peptide with Arg2 mutated to Ala, Arg2 is central to substrate recognition also in LSD2 Mus musculus [histone H3 peptide 21mer]-L-lysine4 + succinate + formaldehyde + CO2
-
 ?
[histone H3 peptide 21mer]-N6-methyl-L-lysine4-acetyl-L-lysine9 + 2-oxoglutarate + O2
-
 Mus musculus [histone H3 peptide 21mer]-L-lysine4-acetyl-L-lysine9 + succinate + formaldehyde + CO2
-
 ?
[histone H3 peptide 21mer]-N6-methyl-L-lysine4-methyl-L-arginine17 + 2-oxoglutarate + O2
-
 Mus musculus ?
-
 ?
[histone H3 peptide 21mer]-N6-methyl-L-lysine4-methyl-L-lysine9 + 2-oxoglutarate + O2
-
 Mus musculus ?
-
 ?
[histone H3 peptide 30mer]-N6-methyl-L-lysine4 + 2-oxoglutarate + O2
-
 Mus musculus [histone H3 peptide 30mer]-L-lysine4 + succinate + formaldehyde + CO2
-
 ?

Synonyms

Synonyms Comment Organism
amine-oxidase flavin-containing domain 1
-
 Mus musculus
AOF1
-
 Mus musculus
LSD2
-
 Mus musculus
lysine-specific demethylase 2
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.004
-
 [histone H3 peptide 21mer]-N6-methyl-L-lysine4-methyl-L-lysine9 pH 8.0, 25°C, recombinant wild-type LSD2 Mus musculus
0.0043
-
 [histone H3 peptide 21mer P16A]-N6-methyl-L-lysine4 pH 8.0, 25°C, recombinant wild-type LSD2 Mus musculus
0.0046
-
 [histone H3 peptide 21mer]-N6-methyl-L-lysine4 pH 8.0, 25°C, recombinant wild-type LSD2 Mus musculus
0.005
-
 [histone H3 peptide 21mer]-N6-methyl-L-lysine4-methyl-L-arginine17 pH 8.0, 25°C, recombinant wild-type LSD2 Mus musculus
0.0058
-
 [histone H3 peptide 21mer]-N6-methyl-L-lysine4-acetyl-L-lysine9 pH 8.0, 25°C, recombinant wild-type LSD2 Mus musculus
0.0068
-
 [histone H3 peptide 30mer]-N6-methyl-L-lysine4 pH 8.0, 25°C, recombinant wild-type LSD2 Mus musculus
0.0222
-
 [histone H3 peptide 21mer]-N6,N6-dimethyl-L-lysine4-dimethyl-L-lysine9 pH 8.0, 25°C, recombinant wild-type LSD2 Mus musculus
0.033
-
 [histone H3 peptide 21mer]-N6-methyl-L-lysine4 pH 8.0, 25°C, recombinant wild-type LSD2 Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Mus musculus

Cofactor

Cofactor Comment Organism Structure
flavin dependent on Mus musculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00015
-
 [histone H3 peptide 21mer]-L-methionine4 pH 8.0, 25°C, recombinant wild-type LSD2 Mus musculus
0.002
-
 [histone H3 peptide 21mer]-L-glutamine4 pH 8.0, 25°C, recombinant wild-type LSD2 Mus musculus
0.0066
-
 [histone H3 peptide 21mer]-N6-methyl-L-lysine9 pH 8.0, 25°C, recombinant wild-type LSD2 Mus musculus
0.033
-
 [histone H3 peptide 21mer]-L-lysine4 pH 8.0, 25°C, recombinant wild-type LSD2 Mus musculus
0.046
-
 [histone H3 peptide 21mer]-L-arginine4 pH 8.0, 25°C, recombinant wild-type LSD2 Mus musculus
0.058
-
 [histone H3 peptide 21mer]-N6,N6,N6-trimethyl-L-lysine4 pH 8.0, 25°C, recombinant wild-type LSD2 Mus musculus