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Literature summary for 1.14.99.63 extracted from

  • Kildegaard, K.R.; Adiego-Perez, B.; Domenech Belda, D.; Khangura, J.K.; Holkenbrink, C.; Borodina, I.
    Engineering of Yarrowia lipolytica for production of astaxanthin (2017), Synth. Syst. Biotechnol., 2, 287-294 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
nutrition engineering of Yarrowia lipolytica for de novo production of the food and feed additive astaxanthin by fermentation. The astaxanthin-producing Yarrowia lipolytica shows great promise for employment in biological astaxanthin production. The genes for beta-carotene biosynthesis: bi-functional phytoene synthase/lycopene cyclase (crtYB) and phytoene desaturase (crtI) from Xanthophyllomyces dendrorhousa are introduced. The activities of 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG1) and geranylgeranyl diphosphate synthase (GGS1/crtE) in the best producing strain and optimized. Downregulation of the competing squalene synthase SQS1 increases the beta-carotene titer. Then a beta-carotene ketolase (crtW) from Paracoccus sp. N81106 and hydroxylase (crtZ) from Pantoea ananatis are introduced to convert beta-carotene into astaxanthin. The constructed strain accumulates 10.4 mg/l of astaxanthin but also accumulates astaxanthin biosynthesis intermediates, 5.7 mg/l canthaxanthin, and 35.3 mg/l echinenone. The copy numbers of crtZ and crtW are optimized to obtain 3.5 mg/g dry cell weight (54.6 mg/l) of astaxanthin in a microtiter plate cultivation Paracoccus sp. N81106

Organism

Organism UniProt Comment Textmining
Paracoccus sp. N81106 P54972
-
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Synonyms

Synonyms Comment Organism
CrtW
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Paracoccus sp. N81106