Literature summary for 1.14.99.63 extracted from

Scaife, M.A.; Burja, A.M.; Wright, P.C.
Characterization of cyanobacterial beta-carotene ketolase and hydroxylase genes in Escherichia coli, and their application for astaxanthin biosynthesis (2009), Biotechnol. Bioeng., 103, 944-955 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli, plasmid pBAD24	Nostoc punctiforme
expression in Escherichia coli, plasmid pBAD24	Synechocystis sp. PCC 6803
expression in Escherichia coli, plasmid pBAD24. Combined overexpression of	Synechocystis sp. PCC 6803

Organism

Organism	UniProt	Comment	Textmining
Nostoc punctiforme	-	-	-
Synechocystis sp. PCC 6803	Q55808	-	-

Synonyms

Synonyms	Comment	Organism
CrtO beta-carotene ketolase	-	Synechocystis sp. PCC 6803
CrtW ketolase	-	Nostoc punctiforme

General Information

General Information	Comment	Organism
metabolism	Synechocystis sp. PCC 6803 is unique in that it contains only a single beta-carotene ketolase protein within its genome. Improved expression of this CrtO ketolase, via application of the pBAD promoter, demonstrates that despite the common reference to CrtO ketolases as mono ketolases, efficient expression can enable enhanced diketo activity	Synechocystis sp. PCC 6803

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Release 2023.1 (January 2023)