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Literature summary for 1.14.99.63 extracted from

  • Lu, Q.; Bu, Y.F.; Liu, J.Z.
    Metabolic engineering of Escherichia coli for producing astaxanthin as the predominant carotenoid (2017), Mar. Drugs, 15, E296 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.99.63

Application

Application Comment Organism
synthesis synthesis of astaxanthin. Astaxanthin is a carotenoid of significant commercial value due to its superior antioxidant potential and wide applications in the aquaculture, food, cosmetic and pharmaceutical industries. The Brevundimonas sp. SD212 crtW and Pantoea ananatis crtZ genes are the best combination for astaxanthin production. After balancing the activities of beta-carotene ketolase and hydroxylase, an Escherichia coli ASTA-1 that carries neither a plasmid nor an antibiotic marker is constructed to produce astaxanthin as the predominant carotenoid (96.6%) with a specific content of 7.4/g dry cell weight without an addition of inducer Brevundimonas sp. NBRC 101024

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Brevundimonas sp. NBRC 101024
expression in Escherichia coli Paracoccus sp. N81106
expression in Escherichia coli Sphingomonas sp. DC18
expression in Eschreichia coli Paracoccus sp. PC1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-carotene + 2 reduced acceptor + 2 O2 Brevundimonas sp. NBRC 101024 beta-carotene ketolase plays an important role in astaxanthin production echinenone + 2 acceptor + 3 H2O
-
 ?
beta-carotene + 2 reduced acceptor + 2 O2 Paracoccus sp. N81106 beta-carotene ketolase plays an important role in astaxanthin production echinenone + 2 acceptor + 3 H2O
-
 ?
beta-carotene + 2 reduced acceptor + 2 O2 Sphingomonas sp. DC18 beta-carotene ketolase plays an important role in astaxanthin production echinenone + 2 acceptor + 3 H2O
-
 ?
beta-carotene + 2 reduced acceptor + 2 O2 Paracoccus sp. PC1 beta-carotene ketolase plays an important role in astaxanthin production echinenone + 2 acceptor + 3 H2O
-
 ?
echinenone + 2 reduced acceptor + 2 O2 Brevundimonas sp. NBRC 101024 beta-carotene ketolase plays an important role in astaxanthin production canthaxanthin + 2 acceptor + 3 H2O
-
 ?
echinenone + 2 reduced acceptor + 2 O2 Paracoccus sp. N81106 beta-carotene ketolase plays an important role in astaxanthin production canthaxanthin + 2 acceptor + 3 H2O
-
 ?
echinenone + 2 reduced acceptor + 2 O2 Sphingomonas sp. DC18 beta-carotene ketolase plays an important role in astaxanthin production canthaxanthin + 2 acceptor + 3 H2O
-
 ?
echinenone + 2 reduced acceptor + 2 O2 Paracoccus sp. PC1 beta-carotene ketolase plays an important role in astaxanthin production canthaxanthin + 2 acceptor + 3 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Brevundimonas sp. NBRC 101024 Q4W8B8
-
-
Paracoccus sp. N81106 P54972 mutant enzyme L175W
-
Paracoccus sp. PC1 Q44261
-
-
Sphingomonas sp. DC18 A3QQZ2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-carotene + 2 reduced acceptor + 2 O2
-
 Brevundimonas sp. NBRC 101024 echinenone + 2 acceptor + 3 H2O
-
 ?
beta-carotene + 2 reduced acceptor + 2 O2
-
 Paracoccus sp. N81106 echinenone + 2 acceptor + 3 H2O
-
 ?
beta-carotene + 2 reduced acceptor + 2 O2
-
 Sphingomonas sp. DC18 echinenone + 2 acceptor + 3 H2O
-
 ?
beta-carotene + 2 reduced acceptor + 2 O2
-
 Paracoccus sp. PC1 echinenone + 2 acceptor + 3 H2O
-
 ?
beta-carotene + 2 reduced acceptor + 2 O2 beta-carotene ketolase plays an important role in astaxanthin production Brevundimonas sp. NBRC 101024 echinenone + 2 acceptor + 3 H2O
-
 ?
beta-carotene + 2 reduced acceptor + 2 O2 beta-carotene ketolase plays an important role in astaxanthin production Paracoccus sp. N81106 echinenone + 2 acceptor + 3 H2O
-
 ?
beta-carotene + 2 reduced acceptor + 2 O2 beta-carotene ketolase plays an important role in astaxanthin production Sphingomonas sp. DC18 echinenone + 2 acceptor + 3 H2O
-
 ?
beta-carotene + 2 reduced acceptor + 2 O2 beta-carotene ketolase plays an important role in astaxanthin production Paracoccus sp. PC1 echinenone + 2 acceptor + 3 H2O
-
 ?
echinenone + 2 reduced acceptor + 2 O2
-
 Brevundimonas sp. NBRC 101024 canthaxanthin + 2 acceptor + 3 H2O
-
 ?
echinenone + 2 reduced acceptor + 2 O2
-
 Paracoccus sp. N81106 canthaxanthin + 2 acceptor + 3 H2O
-
 ?
echinenone + 2 reduced acceptor + 2 O2
-
 Sphingomonas sp. DC18 canthaxanthin + 2 acceptor + 3 H2O
-
 ?
echinenone + 2 reduced acceptor + 2 O2
-
 Paracoccus sp. PC1 canthaxanthin + 2 acceptor + 3 H2O
-
 ?
echinenone + 2 reduced acceptor + 2 O2 beta-carotene ketolase plays an important role in astaxanthin production Brevundimonas sp. NBRC 101024 canthaxanthin + 2 acceptor + 3 H2O
-
 ?
echinenone + 2 reduced acceptor + 2 O2 beta-carotene ketolase plays an important role in astaxanthin production Paracoccus sp. N81106 canthaxanthin + 2 acceptor + 3 H2O
-
 ?
echinenone + 2 reduced acceptor + 2 O2 beta-carotene ketolase plays an important role in astaxanthin production Sphingomonas sp. DC18 canthaxanthin + 2 acceptor + 3 H2O
-
 ?
echinenone + 2 reduced acceptor + 2 O2 beta-carotene ketolase plays an important role in astaxanthin production Paracoccus sp. PC1 canthaxanthin + 2 acceptor + 3 H2O
-
 ?

Synonyms

Synonyms Comment Organism
beta-carotene ketolase
-
 Brevundimonas sp. NBRC 101024
beta-carotene ketolase
-
 Paracoccus sp. N81106
beta-carotene ketolase
-
 Sphingomonas sp. DC18
beta-carotene ketolase
-
 Paracoccus sp. PC1
CrtW
-
 Brevundimonas sp. NBRC 101024
CrtW
-
 Paracoccus sp. N81106
CrtW
-
 Sphingomonas sp. DC18
CrtW
-
 Paracoccus sp. PC1

General Information

General Information Comment Organism
metabolism beta-carotene ketolase plays an important role in astaxanthin production Brevundimonas sp. NBRC 101024
metabolism beta-carotene ketolase plays an important role in astaxanthin production Paracoccus sp. N81106
metabolism beta-carotene ketolase plays an important role in astaxanthin production Sphingomonas sp. DC18
metabolism beta-carotene ketolase plays an important role in astaxanthin production Paracoccus sp. PC1