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Literature summary for 1.14.99.56 extracted from

  • Frandsen, K.E.; Simmons, T.J.; Dupree, P.; Poulsen, J.C.; Hemsworth, G.R.; Ciano, L.; Johnston, E.M.; Tovborg, M.; Johansen, K.S.; von Freiesleben, P.; Marmuse, L.; Fort, S.; Cottaz, S.; Driguez, H.; Henrissat, B.; Lenfant, N.; Tuna, F.; Baldansuren, A.; Davies, G.J.; Lo Leggio, L.; Walton, P.H.
    The molecular basis of polysaccharide cleavage by lytic polysaccharide monooxygenases (2016), Nat. Chem. Biol., 12, 298-303.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Aspergillus oryzae Panus similis

Crystallization (Commentary)

Crystallization (Comment) Organism
substrate cellohexaose binds to subsites ?4 to +2, and cellotriose from subsites -1 to +2. Residue Tyr203 is placed approximately 15 A away from the copper active site Panus similis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.049
-
 cellotetraose pH 7.0, 37°C Panus similis

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ residue Tyr203 is placed approximately 15 A away from the copper active site Panus similis

Organism

Organism UniProt Comment Textmining
Panus similis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellohexaose + acceptor + O2
-
 Panus similis oxidized cellobiose and cellotriose + reduced acceptor + H2O
-
 ?
cellotetraose + acceptor + O2
-
 Panus similis ? + reduced acceptor + H2O
-
 ?
cellotriose + acceptor + O2
-
 Panus similis ? + reduced acceptor + H2O
-
 ?
phosphoric acid swollen cellulose + acceptor + O2
-
 Panus similis oxidized cellobiose and cellotriose + reduced acceptor + H2O main products, C4 is the sole site of oxidation ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.11
-
 cellotetraose pH 7.0, 37°C Panus similis