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Literature summary for 1.14.99.54 extracted from

  • Forsberg, Z.; Bissaro, B.; Gullesen, J.; Dalhus, B.; Vaaje-Kolstad, G.; Eijsink, V.G.H.
    Structural determinants of bacterial lytic polysaccharide monooxygenase functionality (2018), J. Biol. Chem., 293, 1397-1412 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.99.54

Cloned(Commentary)

Cloned (Comment) Organism
-
 Streptomyces coelicolor
-
 Micromonospora aurantiaca

Crystallization (Commentary)

Crystallization (Comment) Organism
structure of the catalytic domain, residues 37-230, to 1.08 A resolution. The active site in is formed by residues His-37 and His-144 that coordinate the copper atom in a T-shaped geometry Streptomyces coelicolor
structure of the catalytic domain, residues 37-230, to 1.08 A resolution. The active site in is formed by residues His-37 and His-144 that coordinate the copper atom in a T-shaped geometry Micromonospora aurantiaca

Protein Variants

Protein Variants Comment Organism
A148G mutation leads to loss of C4 oxidation, i.e to the activity of EC 1.14.99.54 Streptomyces coelicolor
A148S mutation leads to loss of C4 oxidation, i.e to the activity of EC 1.14.99.54 Streptomyces coelicolor
D140A mutant shows moderately reduced activity and essentially unchanged oxidative regioselectivity Micromonospora aurantiaca
additional information neither truncation of the LPMO10B family 2 carbohydrate-binding module nor mutations altering access to the solvent-exposed axial copper coordination site significantly change the C1:C4 oxidation ratio Micromonospora aurantiaca
N85F mutation changes the C1:C4 oxidation ratio from 0.9 (for the wild-type) to 5.9 Micromonospora aurantiaca
W82Y mutation changes the C1:C4 oxidation ratio from 0.9 (for the wild-type) to 2.0 Micromonospora aurantiaca
W82Y/N85F mutation changes the C1:C4 oxidation ratio from 0.9 (for the wild-type) to 10.9 Micromonospora aurantiaca
W82Y/N85F/Q141W mutation changes the C1:C4 oxidation ratio from 0.9 (for the wild-type) to 5.1 Micromonospora aurantiaca
W82Y/N85F/Y116F mutation changes the C1:C4 oxidation ratio from 0.9 (for the wild-type) to 14.7 Micromonospora aurantiaca
W82Y/N85F/Y116F/Q141W mutation changes the C1:C4 oxidation ratio from 0.9 (for the wild-type) to 5.8 Micromonospora aurantiaca
W88Y/N91F mutation leads to loss of C4 oxidation, i.e to the activity of EC 1.14.99.54 Streptomyces coelicolor
Y116F no changes in the C1:C4 oxidation ratio Micromonospora aurantiaca

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+
-
 Streptomyces coelicolor
Cu2+ the active site in is formed by residues His-37 and His-144 that coordinate the copper atom in a T-shaped geometry Micromonospora aurantiaca

Organism

Organism UniProt Comment Textmining
Micromonospora aurantiaca D9SZQ3
-
-
Micromonospora aurantiaca DSM 43813 D9SZQ3
-
-
Streptomyces coelicolor Q9RJC1
-
-
Streptomyces coelicolor ATCC BAA-471 Q9RJC1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + ascorbic acid + O2
-
 Streptomyces coelicolor ? + dehydroascorbate + H2O
-
 ?
chitin + ascorbic acid + O2
-
 Micromonospora aurantiaca ? + dehydroascorbate + H2O
-
 ?
chitin + ascorbic acid + O2
-
 Streptomyces coelicolor ATCC BAA-471 ? + dehydroascorbate + H2O
-
 ?
chitin + ascorbic acid + O2
-
 Micromonospora aurantiaca DSM 43813 ? + dehydroascorbate + H2O
-
 ?
phosphoric acid swollen cellulase + ascorbic acid + O2
-
 Streptomyces coelicolor ? + dehydroascorbate + H2O
-
 ?
phosphoric acid swollen cellulase + ascorbic acid + O2
-
 Micromonospora aurantiaca ? + dehydroascorbate + H2O
-
 ?
phosphoric acid swollen cellulase + ascorbic acid + O2
-
 Streptomyces coelicolor ATCC BAA-471 ? + dehydroascorbate + H2O
-
 ?
phosphoric acid swollen cellulase + ascorbic acid + O2
-
 Micromonospora aurantiaca DSM 43813 ? + dehydroascorbate + H2O
-
 ?

Synonyms

Synonyms Comment Organism
chitin-binding domain 3 protein
-
 Micromonospora aurantiaca
LPMO10B
-
 Streptomyces coelicolor
LPMO10B
-
 Micromonospora aurantiaca
Micau_1630
-
 Micromonospora aurantiaca
SCO0643
-
 Streptomyces coelicolor