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Literature summary for 1.14.99.54 extracted from

  • Nekiunaite, L.; Petrovic, D.M.; Westereng, B.; Vaaje-Kolstad, G.; Hachem, M.A.; Varnai, A.; Eijsink, V.G.
    FgLPMO9A from Fusarium graminearum cleaves xyloglucan independently of the backbone substitution pattern (2016), FEBS Lett., 590, 3346-3356 .
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Fusarium graminearum I1REU9
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Fusarium graminearum ATCC MYA-4620 I1REU9
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme catalyzes mixed C1/C4 oxidative cleavage of cellulose, reactions of EC 1.14.99.54 and EC1.14.99.56, and xyloglucan, reaction of lytic xyloglucan monooxygenase, but is inactive toward other (1,4)-linked beta-glucans or chitin and cellooligosaccharides with a degree of polymerization DP 3-6. It shows broad specificity on xyloglucan, cleaving any glycosidic bond in the beta-glucan main chain, regardless of xylosyl substitutions. When incubated with a mixture of xyloglucan and cellulose, LPMO9A efficiently attacks the xyloglucan, whereas cellulose conversion is inhibited. no substrates: xyloglucan-heptamer, birchwood xylan, wheat arabinoxylan, konjac glucomannan, ivory nut mannan, beta-glucan from barley, lichenan from Icelandic moss, starch, and spruce galactoglucomannan Fusarium graminearum ?
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?
additional information enzyme catalyzes mixed C1/C4 oxidative cleavage of cellulose, reactions of EC 1.14.99.54 and EC1.14.99.56, and xyloglucan, reaction of lytic xyloglucan monooxygenase, but is inactive toward other (1,4)-linked beta-glucans or chitin and cellooligosaccharides with a degree of polymerization DP 3-6. It shows broad specificity on xyloglucan, cleaving any glycosidic bond in the beta-glucan main chain, regardless of xylosyl substitutions. When incubated with a mixture of xyloglucan and cellulose, LPMO9A efficiently attacks the xyloglucan, whereas cellulose conversion is inhibited. no substrates: xyloglucan-heptamer, birchwood xylan, wheat arabinoxylan, konjac glucomannan, ivory nut mannan, beta-glucan from barley, lichenan from Icelandic moss, starch, and spruce galactoglucomannan Fusarium graminearum ATCC MYA-4620 ?
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?
phosphoric acid swollen cellulose + ascorbic acid + O2
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Fusarium graminearum ? + dehydroascorbic acid + H2O
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?
phosphoric acid swollen cellulose + ascorbic acid + O2
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Fusarium graminearum ATCC MYA-4620 ? + dehydroascorbic acid + H2O
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?
reduced xyloglucan oligosaccharide + ascorbic acid + O2 pure xyloglucan oligosaccharide with DP14 Fusarium graminearum xyloglucan oligosaccharides + dehydroascorbic acid + H2O
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?
reduced xyloglucan oligosaccharide + ascorbic acid + O2 pure xyloglucan oligosaccharide with DP14 Fusarium graminearum ATCC MYA-4620 xyloglucan oligosaccharides + dehydroascorbic acid + H2O
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?
tamarind xyloglucan + ascorbic acid + O2
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Fusarium graminearum ? + dehydroascorbic acid + H2O
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?
tamarind xyloglucan + ascorbic acid + O2
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Fusarium graminearum ATCC MYA-4620 ? + dehydroascorbic acid + H2O
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?

Synonyms

Synonyms Comment Organism
FG02202.1
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Fusarium graminearum
LPMO9A
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Fusarium graminearum

Cofactor

Cofactor Comment Organism Structure
additional information in the absence of electron donor, such as ascorbic acid, no reaction is detected Fusarium graminearum