Literature summary for 1.14.99.54 extracted from

Tanghe, M.; Danneels, B.; Last, M.; Beerens, K.; Stals, I.; Desmet, T.
Disulfide bridges as essential elements for the thermostability of lytic polysaccharide monooxygenase LPMO10C from Streptomyces coelicolor (2017), Protein Eng. Des. Sel., 30, 401-408.

Search for more literature for 1.14.99.54

Protein Variants

Protein Variants	Comment	Organism
A143C/P183C/S73C/A115C	introduction of two additional disulfide bridges, mutant displays a 12°C increase in melting temperature and is able to retain 60% of its activity after heat treatment	Streptomyces coelicolor
additional information	introduction of additional disulfide bridges. Four out of 16 variants display an improvement in melting temperature, ranging from 2°C to 9°C	Streptomyces coelicolor
S73C/A115C	introduction of an additional disulfide bridge, mutant displays a 9°C increase in melting temperature	Streptomyces coelicolor

Organism

Organism	UniProt	Comment	Textmining
Streptomyces coelicolor	Q9RJY2	-	-
Streptomyces coelicolor ATCC BAA-471	Q9RJY2	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
additional information	when the three disulfide bridges of the protein are broken, irreversible unfolding occurs and no residual activity can be detected after a heat treatment at 80°C	Streptomyces coelicolor

Synonyms

Synonyms	Comment	Organism
LPMO10C	-	Streptomyces coelicolor
SCO1188	-	Streptomyces coelicolor

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
51	-	melting temperature, wild-type	Streptomyces coelicolor
60	-	melting temperature, mutant S73C/A115C	Streptomyces coelicolor
63	-	melting temperature, mutant A143C/P183C/S73C/A115C	Streptomyces coelicolor
80	-	2 h, 34% residual activity. When the three disulfide bridges of the protein are broken, irreversible unfolding occurs and no residual activity can be detected after a similar heat treatment	Streptomyces coelicolor

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)