Application | Comment | Organism |
---|---|---|
analysis | assay based on adsorption of the fluorescent dye SYTO-62, adapted to identify and localize LPMO-catalyzed formation of carboxyl groups on a cellulose surface model substrate which provides amorphous and crystalline regions alternating on a nano-flat cellulose surface | Neurospora crassa |
Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Neurospora crassa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | Q1K4Q1 | - |
- |
Neurospora crassa DSM 1257 | Q1K4Q1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
avicel + ascorbate + O2 | - |
Neurospora crassa | ? + dehydroascorbate + H2O | - |
? | |
avicel + ascorbate + O2 | - |
Neurospora crassa DSM 1257 | ? + dehydroascorbate + H2O | - |
? | |
cellulose + ascorbate + O2 | - |
Neurospora crassa | C1-oxidized cellooligosaccharides + dehydroascorbate + H2O | - |
? | |
cellulose + ascorbate + O2 | - |
Neurospora crassa DSM 1257 | C1-oxidized cellooligosaccharides + dehydroascorbate + H2O | - |
? |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme introduces carboxyl groups primarily in surface-exposed crystalline areas of the cellulosic substrate. Cellulose nano-fibrils exposed on the surface are degraded into shorter and thinner insoluble fragments. Prior action of LPMO enables cellulases to attack otherwise highly resistant crystalline substrate areas and promotes an overall faster and more complete surface degradation | Neurospora crassa |