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Literature summary for 1.14.99.53 extracted from

  • Stopamo, F.; Rohr, A.; Mekasha, S.; Petrovi, D.; Varnai, A.; Eijsink, V.
    Characterization of a lytic polysaccharide monooxygenase from Aspergillus fumigatus shows functional variation among family AA11 fungal LPMOs (2021), J. Biol. Chem., 297, 101421.
    View publication on PubMed
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Activating Compound

Activating Compound Comment Organism Structure
H2O2 catalytic activity of AA11A on chitin increases in presence of H2O2 Aspergillus fumigatus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Aspergillus fumigatus

Crystallization (Commentary)

Crystallization (Comment) Organism
structure shows a substrate-binding surface with features similar to known chitin-active LPMOs and the absence of a carbohydrate-binding module Aspergillus fumigatus

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus Q4WF00
-
-
Aspergillus fumigatus ATCC MYA-4609 Q4WF00
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein sequence contains a possible N-glycosylation site, at Asn62. Treatment with endo-beta-N-acetylglucosaminidase leads to a mass reduction to 22 kDA Aspergillus fumigatus

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-chitin + gallate + O2
-
 Aspergillus fumigatus chito-oligosaccharide C1-aldonic acids + dehydroascorbate + H2O
-
 ?
alpha-chitin + gallate + O2
-
 Aspergillus fumigatus ATCC MYA-4609 chito-oligosaccharide C1-aldonic acids + dehydroascorbate + H2O
-
 ?
beta-chitin + ascorbate + O2
-
 Aspergillus fumigatus ? + dehydroascorbate + H2O
-
 ?
beta-chitin + ascorbate + O2
-
 Aspergillus fumigatus ATCC MYA-4609 ? + dehydroascorbate + H2O
-
 ?
additional information presence of ascorbate is required. No substrates: phosphoric acid-swollen cellulose, avicel, tamarind xyloglucan, birchwood xylan, beechwood xylan, acetyl glucuronoxylan from aspen, ivory nut mannan, acetylated konjac glucomannan, potato starch, heparin, hyaluronic acid, and chitosan Aspergillus fumigatus ?
-
 ?
additional information presence of ascorbate is required. No substrates: phosphoric acid-swollen cellulose, avicel, tamarind xyloglucan, birchwood xylan, beechwood xylan, acetyl glucuronoxylan from aspen, ivory nut mannan, acetylated konjac glucomannan, potato starch, heparin, hyaluronic acid, and chitosan Aspergillus fumigatus ATCC MYA-4609 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE, x * 21750, calculated from sequence Aspergillus fumigatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Aspergillus fumigatus