Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.99.53 extracted from

  • Ma, L.; Liu, Z.; Kong, Z.; Wang, M.; Li, T.; Zhu, H.; Wan, Q.; Liu, D.; Shen, Q.
    Functional characterization of a novel copper-dependent lytic polysaccharide monooxygenase TgAA11 from Trichoderma guizhouense NJAU 4742 in the oxidative degradation of chitin (2021), Carbohydr. Polym., 258, 117708.
    View publication on PubMed
Search for more literature for 1.14.99.53

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Trichoderma guizhouense

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling. Residues His1 and His61 directly coordinate the basic copper cofactor in T-shaped geometry. The axial, noncoordinating active site residue is Tyr142 Trichoderma guizhouense

Protein Variants

Protein Variants Comment Organism
H1A hydrogen bonds between the copper ion and the predicted active site residues are similar to wild-type Trichoderma guizhouense
H61A distance between the copper ion and the predicted active site residue are increased Trichoderma guizhouense
Y142A distance between the copper ion and the predicted active site residue are increased Trichoderma guizhouense

Metals/Ions

Metals/Ions Comment Organism Structure
copper tight binding activity toward Cu2+ , Kd value 0.0048 mM. Residues His1 and His61 directly coordinate the basic copper cofactor in T-shaped geometry Trichoderma guizhouense
additional information no binding activity toward Zn2+ Trichoderma guizhouense

Organism

Organism UniProt Comment Textmining
Trichoderma guizhouense A0A1T3C909 cf. EC 1.14.99.54
-
Trichoderma guizhouense NJAU 47420 A0A1T3C909 cf. EC 1.14.99.54
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-chitin + ascorbate + O2
-
 Trichoderma guizhouense ? + dehydroascorbate + H2O
-
 ?
alpha-chitin + ascorbate + O2
-
 Trichoderma guizhouense NJAU 47420 ? + dehydroascorbate + H2O
-
 ?
alpha-chitin + gallate + O2
-
 Trichoderma guizhouense ? + H2O
-
 ?
alpha-chitin + gallate + O2
-
 Trichoderma guizhouense NJAU 47420 ? + H2O
-
 ?
beta-chitin + ascorbate + O2
-
 Trichoderma guizhouense ? + dehydroascorbate + H2O
-
 ?
beta-chitin + ascorbate + O2
-
 Trichoderma guizhouense NJAU 47420 ? + dehydroascorbate + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Trichoderma guizhouense

Synonyms

Synonyms Comment Organism
AA11
-
 Trichoderma guizhouense