BRENDA - Enzyme Database show
show all sequences of 1.14.99.53

A novel expression system for lytic polysaccharide monooxygenases

Courtade, G.; Le, S.; Satrom, G.; Brautaset, T.; Aachmann, F.; Carbohydr. Res. 448, 212-219 (2017)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
construction of a cassette vector containing the XylS/Pm system that can easily be used for exchanging LPMO coding genes with or without signal sequences. The cassette reliably produces mature (translocated) LPMOs under controlled conditions. The signal sequence of LPMO10A from Serratia marcescens gives highest levels of recombinant protein production and translocation
Serratia marcescens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Serratia marcescens
-
-
-
Application (protein specific)
Application
Commentary
Organism
synthesis
construction of a cassette vector containing the XylS/Pm system that can easily be used for exchanging LPMO coding genes with or without signal sequences. The cassette reliably produces mature (translocated) LPMOs under controlled conditions. The signal sequence of LPMO10A from Serratia marcescens gives highest levels of recombinant protein production and translocation
Serratia marcescens
Other publictions for EC 1.14.99.53
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744375
Bissaro
How a lytic polysaccharide mo ...
Serratia marcescens
Biochemistry
57
1893-1906
2018
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3
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745380
Forsberg
Structural determinants of ba ...
Micromonospora aurantiaca, Micromonospora aurantiaca DSM 43813, Streptomyces coelicolor, Streptomyces coelicolor ATCC BAA-471
J. Biol. Chem.
293
1397-1412
2018
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2
2
12
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2
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12
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8
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740090
Bacik
Neutron and atomic resolution ...
Jonesia denitrificans, Jonesia denitrificans DSM 20603
Biochemistry
56
2529-2532
2017
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1
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1
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4
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4
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743966
ODell
Crystallization of a fungal l ...
Neurospora crassa
Acta Crystallogr. Sect. F
73
70-78
2017
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1
1
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743999
Yang
Improving extracellular produ ...
Serratia marcescens
AMB Express
7
170
2017
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2
1
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744034
ODell
Oxygen activation at the acti ...
Neurospora crassa
Angew. Chem. Int. Ed. Engl.
56
767-770
2017
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1
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744364
Bacik
Neutron and atomic resolution ...
Jonesia denitrificans, Jonesia denitrificans DSM 20603
Biochemistry
56
2529-2532
2017
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1
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1
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4
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744629
Frandsen
Unliganded and substrate boun ...
Lentinus similis
Carbohydr. Res.
448
187-190
2017
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1
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1
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744630
Valenzuela
Fast purification method of f ...
Streptomyces ambofaciens
Carbohydr. Res.
448
205-211
2017
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1
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1
1
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1
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744631
Courtade
A novel expression system for ...
Serratia marcescens
Carbohydr. Res.
448
212-219
2017
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1
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740345
Gregory
Activity, stability and 3-D st ...
Bacillus amyloliquefaciens, Bacillus amyloliquefaciens DSM 7
Dalton Trans.
45
16904-16912
2016
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1
1
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2
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2
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4
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2
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740398
Yu
Overproduction and characteriz ...
Bacillus atrophaeus 1942, Bacillus atrophaeus
Enzyme Microb. Technol.
93-94
150-156
2016
-
1
1
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1
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2
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1
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2
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1
1
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1
1
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740455
Mekasha
Structural and functional char ...
Jonesia denitrificans, Jonesia denitrificans DSM 20603
FEBS Lett.
590
34-42
2016
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1
1
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1
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4
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6
1
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6
1
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740758
Forsberg
Structural and functional anal ...
Cellvibrio japonicus
J. Biol. Chem.
291
7300-7312
2016
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1
1
1
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1
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1
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2
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1
1
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741377
Loose
Activation of bacterial lytic ...
Streptomyces coelicolor, Streptomyces coelicolor ATCC BAA-471
Protein Sci.
25
2175-2186
2016
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1
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3
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6
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6
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1
1
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736250
Zhang
Expression and characterizatio ...
Bacillus thuringiensis, Bacillus thuringiensis ACCC 10066, Bacillus thuringiensis serovar kurstaki, Bacillus thuringiensis serovar kurstaki ACCC 10066
Int. J. Biol. Macromol.
79
72-75
2015
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2
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1
2
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4
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1
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1
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12
1
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740261
Hamre
Activation of enzymatic chitin ...
Serratia marcescens
Carbohydr. Res.
407
166-169
2015
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1
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1
1
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740434
Nakagawa
A small lytic polysaccharide m ...
Streptomyces griseus subsp. griseus, Streptomyces griseus subsp. griseus JCM 4626
FEBS J.
282
1065-1079
2015
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1
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1
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2
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4
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1
1
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740439
Paspaliari
Listeria monocytogenes has a f ...
Listeria monocytogenes serotype 1/2a, Listeria monocytogenes serotype 1/2a ATCC BAA-679
FEBS J.
282
921-936
2015
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8
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1
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736451
Gudmundsson
Structural and electronic snap ...
Enterococcus faecalis
J. Biol. Chem.
289
18782-18792
2014
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1
1
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1
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740205
Book
Evolution of substrate specifi ...
Burkholderia pseudomallei 1710b, Burkholderia pseudomallei, Enterococcus faecalis, Enterococcus faecalis ATCC 700802
Biotechnol. Biofuels
7
109
2014
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2
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740448
Loose
A rapid quantitative activity ...
Vibrio cholerae
FEBS Lett.
588
3435-3440
2014
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1
1
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1
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1
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741038
Hemsworth
Discovery and characterization ...
Aspergillus oryzae, Aspergillus oryzae ATCC 42149
Nat. Chem. Biol.
10
122-126
2014
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1
1
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741336
Forsberg
Structural and functional char ...
Streptomyces coelicolor, Streptomyces coelicolor ATCC BAA-471
Proc. Natl. Acad. Sci. USA
111
8446-8451
2014
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1
1
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1
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5
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740562
Nakagawa
Conversion of alpha-chitin sub ...
Serratia marcescens
J. Agric. Food Chem.
61
11061-11066
2013
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1
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1
1
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736633
Vaaje-Kolstad
Characterization of the chitin ...
Enterococcus faecalis
J. Mol. Biol.
416
239-254
2012
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741326
Aachmann
NMR structure of a lytic polys ...
Serratia marcescens
Proc. Natl. Acad. Sci. USA
109
18779-18784
2012
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737264
Vaaje-Kolstad
An oxidative enzyme boosting t ...
Serratia marcescens
Science
330
219-222
2010
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707459
Duzhak
Extracellular chitinases of mu ...
Serratia marcescens
Biochemistry (Moscow)
74
209-214
2009
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