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Literature summary for 1.14.99.53 extracted from

  • Mekasha, S.; Forsberg, Z.; Dalhus, B.; Bacik, J.P.; Choudhary, S.; Schmidt-Dannert, C.; Vaaje-Kolstad, G.; Eijsink, V.G.
    Structural and functional characterization of a small chitin-active lytic polysaccharide monooxygenase domain of a multi-modular chitinase from Jonesia denitrificans (2016), FEBS Lett., 590, 34-42.
    View publication on PubMed
Search for more literature for 1.14.99.53

Cloned(Commentary)

Cloned (Comment) Organism
codon-optimized expression in Escherichia coli Jonesia denitrificans

Crystallization (Commentary)

Crystallization (Comment) Organism
1.55 A resolution structure of N-terminal LPMO10A module reveals deletions of interacting loops that protrude from the core beta-sandwich scaffold in larger LPMO10s Jonesia denitrificans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15500
-
-
 Jonesia denitrificans

Organism

Organism UniProt Comment Textmining
Jonesia denitrificans C7R4I0 protein comprises a small N-terminal LPMO10 module named LPMO10A followed by a family 5/12 CBM and a C-terminal GH18 module
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Jonesia denitrificans DSM 20603 C7R4I0 protein comprises a small N-terminal LPMO10 module named LPMO10A followed by a family 5/12 CBM and a C-terminal GH18 module
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-chitin + ascorbate + O2
-
 Jonesia denitrificans C1-oxidized chitooligosaccharides + dehydroascorbate + H2O products are C1-oxidized chitooligomers, mainly tetramers and hexamers ?
alpha-chitin + ascorbate + O2
-
 Jonesia denitrificans DSM 20603 C1-oxidized chitooligosaccharides + dehydroascorbate + H2O products are C1-oxidized chitooligomers, mainly tetramers and hexamers ?
beta-chitin + ascorbate + O2 beta-chitin is preferred over alpha-chitin Jonesia denitrificans C1-oxidized chitooligosaccharides + dehydroascorbate + H2O products are C1-oxidized chitooligomers, mainly tetramers and hexamers ?
beta-chitin + ascorbate + O2 beta-chitin is preferred over alpha-chitin Jonesia denitrificans DSM 20603 C1-oxidized chitooligosaccharides + dehydroascorbate + H2O products are C1-oxidized chitooligomers, mainly tetramers and hexamers ?
additional information the enzyme is active on alpha- and beta-chitin, and the chitin-binding surface previously described for larger LPMOs is fully conserved Jonesia denitrificans ?
-
 ?
additional information the enzyme is active on alpha- and beta-chitin, and the chitin-binding surface previously described for larger LPMOs is fully conserved Jonesia denitrificans DSM 20603 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 15500, N-terminal LPMO10A module Jonesia denitrificans

Synonyms

Synonyms Comment Organism
Jden_1381
-
 Jonesia denitrificans
LPMO10A
-
 Jonesia denitrificans