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Literature summary for 1.14.99.53 extracted from
- Overproduction and characterization of a lytic polysaccharide monooxygenase in Bacillus subtilis using an assay based on ascorbate consumption (2016), Enzyme Microb. Technol., 93-94, 150-156.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | development of a chitinase-coupled assay method and an assay based on consumption of ascorbate. There is no H2O2 production in the presence of colloidal chitin at 4 mg/ml | Bacillus atrophaeus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Baciluus subtilis | Bacillus atrophaeus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | enzyme activity is decreased by NaCl | Bacillus atrophaeus |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus atrophaeus | A0A0H3E2X6 | - |
- |
| Bacillus atrophaeus 1942 | A0A0H3E2X6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| expression Bacillus subtilis yields a simple purification process without complicated periplasmic fractionation, as well as improved productivity | Bacillus atrophaeus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| colloidal chitin + ascorbate + O2 | - |
Bacillus atrophaeus | [chitin oligosaccharide]-N-acetyl-D-glucosaminate + [chitin oligosaccharide]-N-acetyl-D-glucosamino-1,5-lactone + dehydroascorbate + H2O | - |
? | |
| colloidal chitin + ascorbate + O2 | - |
Bacillus atrophaeus 1942 | [chitin oligosaccharide]-N-acetyl-D-glucosaminate + [chitin oligosaccharide]-N-acetyl-D-glucosamino-1,5-lactone + dehydroascorbate + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BATR1942_08650 | - |
Bacillus atrophaeus |
| LPMO10 | - |
Bacillus atrophaeus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
Tris/HCl buffer | Bacillus atrophaeus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
ammonium acetate buffer, 34% of maximum activity | Bacillus atrophaeus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | LPMO10 and a chitinase mutually enhance each otherยs activities upon degrading chitin as the substrate | Bacillus atrophaeus |
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