Any feedback?
Literature summary for 1.14.99.53 extracted from
- Activity, stability and 3-D structure of the Cu(II) form of a chitin-active lytic polysaccharide monooxygenase from Bacillus amyloliquefaciens (2016), Dalton Trans., 45, 16904-16912.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Bacillus amyloliquefaciens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| analysis of the copper active site | Bacillus amyloliquefaciens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | Cu(II) binds with KD value 43 nM at pH 5. The coordination geometry around the copper is distorted from axial symmetry | Bacillus amyloliquefaciens |  |
| Zn2+ | may substitue for Cu2+. KD value 0.0081 mM at pH 5 | Bacillus amyloliquefaciens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus amyloliquefaciens | - |
- |
- |
| Bacillus amyloliquefaciens DSM 7 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| improved expression and purification protocol using Champion pET-SUMO vector, yielding high purity AA10 with yields in excess of 9 mg of protein per litre of culture | Bacillus amyloliquefaciens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-chitin + acceptor + O2 | - |
Bacillus amyloliquefaciens | oligosaccharide aldonic acids + reduced acceptor + H2O | - |
? | |
| alpha-chitin + acceptor + O2 | - |
Bacillus amyloliquefaciens DSM 7 | oligosaccharide aldonic acids + reduced acceptor + H2O | - |
? | |
| beta-chitin + acceptor + O2 | beta chitin from squid pen, best substrate | Bacillus amyloliquefaciens | oligosaccharide aldonic acids + reduced acceptor + H2O | strong preference towards even-numbered products | ? | |
| beta-chitin + acceptor + O2 | beta chitin from squid pen, best substrate | Bacillus amyloliquefaciens DSM 7 | oligosaccharide aldonic acids + reduced acceptor + H2O | strong preference towards even-numbered products | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AA10 | - |
Bacillus amyloliquefaciens |
| RBAM17540 | - |
Bacillus amyloliquefaciens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
chitin binding increases the thermal stability of the enzyme by 8.3 degrees, copper binding increases melting temperature by 21.6 degrees. Addition of chitin to the copper-bound enzyme increases thermal stability by additional 3.5 degrees | Bacillus amyloliquefaciens |
| 68.7 | - |
melting temperature, presence of both copper and chitin | Bacillus amyloliquefaciens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2026.1 (March 2026)
Legal
Curated at
Member of
![DSMZ Digital Diversity]()
![Global Core Biodata Resource]()
![ELIXIR Core Data Resource]()
![German Network for Bioinformatics Infrastructure]()
