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Literature summary for 1.14.99.52 extracted from

  • Castellano, I.; Migliaccio, O.; D'Aniello, S.; Merlino, A.; Napolitano, A.; Palumbo, A.
    Shedding light on ovothiol biosynthesis in marine metazoans (2016), Sci. Rep., 6, 21056 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.99.52

Cloned(Commentary)

Cloned (Comment) Organism
gene ovoA, multi-exonic gene with 19 exons, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real time PCR enzyme expression analysis Paracentrotus lividus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required, the DinB superfamily domain contains the conserved HX3HXE putative iron-binding motif Paracentrotus lividus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-histidine + L-cysteine + O2 Paracentrotus lividus
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 S-(L-histidin-5-yl)-L-cysteine S-oxide + H2O
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 ?

Organism

Organism UniProt Comment Textmining
Paracentrotus lividus A0A140GAV9 collected in the Gulf of Naples, near Castel dell'Ovo, from a location that is not privately-owned or protected in any way, and in collected at the Marine Protected Area Gaiola at station G1 in October of 2013
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Source Tissue

Source Tissue Comment Organism Textmining
egg sea urchin Paracentrotus lividus eggs contain high concentrations (in the mM range) of ovothiol A Paracentrotus lividus
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embryo PlOvoA embryo expression significantly increased at the pluteus stage Paracentrotus lividus
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additional information enzyme expression analysis, PlOvoA expression profile and response to environmental stress conditions, overview. Ovothiol levels significantly increase at the prism stage after exposure to the metal, with respect to the control, whereas no significant increase is detected at the swimming blastula stage Paracentrotus lividus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-histidine + L-cysteine + O2
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 Paracentrotus lividus S-(L-histidin-5-yl)-L-cysteine S-oxide + H2O
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 ?

Synonyms

Synonyms Comment Organism
OvoA
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 Paracentrotus lividus
PlOvoA
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 Paracentrotus lividus

Expression

Organism Comment Expression
Paracentrotus lividus at the pluteus stage PlOvoA mRNA is strongly downregulated after exposure to 0.005-0.010 mM Cd2+ down
Paracentrotus lividus PlOvoA embryo expression significantly increases at the pluteus stage and is upregulated by metals (Cd2+ and Mn2+) at concentrations mimicking polluted sea-water and by cyclic toxic algal blooms, leading to ovothiol biosynthesis. In silico analysis of the PlOvoA upstream region reveals metal and stress responsive elements. PlOvoA expression profile and response to environmental stress conditions, overview. A significant increase of PlOvoA mRNA is observed at the swimming blastula stage after exposure to 0.001-0.005 mM Cd2+, whereas at the early blastula and prism stages, the gene transcription is unaffected. On the contrary, at the pluteus stage PlOvoA mRNA is strongly down-regulated after exposure to 0.005-0.010 mM Cd2+. In the presence of Mn2+, the relative expression ratio of PlOvoA at the swimming blastula stage is close to the minimum value considered significant. No appreciable gene regulation is observed in other developmental stages up

General Information

General Information Comment Organism
evolution OvoA evolved in most marine metazoans and was lost in bony vertebrates during the transition from the aquatic to terrestrial environment. The DinB superfamily domain contains the conserved HX3HXE putative iron-binding motif. The residues 581-587, 602-603, 661-663, and 680, considered to be involved in the formation of SAM-binding site, are conserved with the bacterial orthologous gene. OvoA shares two protein domains with EgtB, the DinB superfamily domain and the FGE-sulfatase domain but differs for the additional C-terminal putative SAM-transferase domain. Evolutionary history of OvoA in metazoans, overview Paracentrotus lividus
metabolism gene ovoA is involved in ovothiol biosynthesis Paracentrotus lividus
additional information enzyme structure modeling, excluding the C-terminal additional domain, overview Paracentrotus lividus
physiological function crucial role of OvoA in protecting embryos released in seawater from environmental cues, thus allowing the survival under different conditions Paracentrotus lividus