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Literature summary for 1.14.99.46 extracted from

  • Kim, K.S.; Pelton, J.G.; Inwood, W.B.; Andersen, U.; Kustu, S.; Wemmer, D.E.
    The Rut pathway for pyrimidine degradation: novel chemistry and toxicity problems (2010), J. Bacteriol., 192, 4089-4102.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.99.46

Cloned(Commentary)

Cloned (Comment) Organism
gene rutA, recombinant overexpression of His-tagged RutA in ASKA strain JW0997 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
thymine + FMNH2 + O2 Escherichia coli
-
 (Z)-2-methylureidoacrylate peracid + FMN
-
 ?
uracil + FMNH2 + O2 Escherichia coli
-
 (Z)-3-ureidoacrylate peracid + FMN
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P75898 gene rutA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged RutA from ASKA strain JW0997 by nickel affinity chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information (Z)-3-ureidoacrylic peracid is not stable and decomposes nonenzymatically to give (Z)-3-ureidoacrylate Escherichia coli ?
-
 ?
thymine + FMNH2 + O2
-
 Escherichia coli (Z)-2-methylureidoacrylate peracid + FMN
-
 ?
uracil + FMNH2 + O2
-
 Escherichia coli (Z)-3-ureidoacrylate peracid + FMN
-
 ?
uracil + FMNH2 + O2 RutA directly cleaves the uracil ring between N-3 and C-4 to yield ureidoacrylate Escherichia coli (Z)-3-ureidoacrylate peracid + FMN product analysis by both NMR spectroscopy and mass spectrometry, overview ?

Synonyms

Synonyms Comment Organism
RutA
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8.2 assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
FAD FAD works much less well than FMN Escherichia coli
FMN preferred cofactor Escherichia coli

General Information

General Information Comment Organism
metabolism RutA has a central role in the Rut pathway, a pyrimidine catabolic pathway, which is composed of seven proteins, all of which are required by Escherichia coli K-12 to grow on uracil as the sole nitrogen source, pathway overview. RutA works in conjunction with the flavin reductase RutF Escherichia coli
physiological function RutA has a central role in the Rut pathway, which is composed of seven proteins, all of which are required by Escherichia coli K-12 to grow on uracil as the sole nitrogen source Escherichia coli