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Literature summary for 1.14.99.46 extracted from

  • Mukherjee, T.; Zhang, Y.; Abdelwahed, S.; Ealick, S.E.; Begley, T.P.
    Catalysis of a flavoenzyme-mediated amide hydrolysis (2010), J. Am. Chem. Soc., 132, 5550-5551.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.99.46

Cloned(Commentary)

Cloned (Comment) Organism
gene rutA, expression of the His-tagged enzyme in Escherichia coli strain BL21, co-expression with rutF Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant RutA, X-ray diffraction structure determination and analysis at 1.8 A resolution Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
thymine + FMNH2 + O2 Escherichia coli
-
 (Z)-2-methylureidoacrylate peracid + FMN
-
 ?
uracil + FMNH2 + O2 Escherichia coli
-
 (Z)-3-ureidoacrylate peracid + FMN
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P75898 gene rutA, encoded in the b1012 operon, containing seven genes, rutA-G
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged RutA from Escherichia coli strain BL21 by nickel affinity chromatography to homogeneity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information (Z)-3-ureidoacrylic peracid decomposes nonenzymatically to form primarily (Z)-3-ureidoacrylic acid and uracil, half life of (Z)-3-ureidoacrylic peracid is 3 hours at pH 10.0 at 25°C and much less at lower pH Escherichia coli ?
-
 ?
thymine + FMNH2 + O2
-
 Escherichia coli (Z)-2-methylureidoacrylate peracid + FMN
-
 ?
uracil + FMNH2 + O2
-
 Escherichia coli (Z)-3-ureidoacrylate peracid + FMN
-
 ?

Synonyms

Synonyms Comment Organism
RutA
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Escherichia coli
additional information RutA-catalyzed reduction or reduction by excess NADH or DTT in the reaction buffer Escherichia coli

General Information

General Information Comment Organism
metabolism RutA is involved in the pyrimidine catabolic Rut pathway, which converts uracil to 3-hydroxypropionate or 2-methyl-3-hydroxypropionate. The flavoenzyme RutA, along with the flavin reductase RutF, catalyzes the initial uracil ring opening by an unprecedented oxidative hydrolysis reaction to give 3-ureidoacrylate Escherichia coli