BRENDA - Enzyme Database show
show all sequences of 1.14.99.38

Cholesterol 25-hydroxylation activity of CYP3A

Honda, A.; Miyazaki, T.; Ikegami, T.; Iwamoto, J.; Maeda, T.; Hirayama, T.; Saito, Y.; Teramoto, T.; Matsuzaki, Y.; J. Lipid Res. 52, 1509-1516 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
recombinant expression in insect cell microsomes via baculovirus transfection; recombinant expression in insect cell microsomes via baculovirus transfection; recombinant expression in insect cell microsomes via baculovirus transfection; recombinant expression in insect cell microsomes via baculovirus transfection
Homo sapiens
Engineering
Amino acid exchange
Commentary
Organism
additional information
in cells overexpressing human recombinant CYP3A4, the activity of cholesterol 25-hydroxylation is higher than that of cholesterol 4beta-hydroxylation, a known marker activity of CYP3A4
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
desmosterol
a potent inhibitor of CH25H; a potent inhibitor of CH25H; a potent inhibitor of CH25H
Homo sapiens
desmosterol
a potent inhibitor of CH25H
Mus musculus
troleandomycin
specific inhibitor of CYP3A
Homo sapiens
troleandomycin
specific inhibitor of CYP3A
Mus musculus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Homo sapiens
-
-
microsome
-
Mus musculus
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
cholesterol + AH2 + O2
Mus musculus
-
25-hydroxycholesterol + A + H2O
-
-
?
cholesterol + AH2 + O2
Homo sapiens
lower 25-hydroxylation enzyme activity by CYP1A2 compared to marker enzyme CYP3A4
25-hydroxycholesterol + A + H2O
-
-
?
cholesterol + AH2 + O2
Homo sapiens
lower 25-hydroxylation enzyme activity by CYP2C9 compared to marker enzyme CYP3A4
25-hydroxycholesterol + A + H2O
-
-
?
cholesterol + AH2 + O2
Homo sapiens
lower 25-hydroxylation enzyme activity by CYP2D6 compared to marker enzyme CYP3A4
25-hydroxycholesterol + A + H2O
-
-
?
cholesterol + AH2 + O2
Homo sapiens
production of 25-hydroxycholesterol by intact CYP3A4, most significant activity is 25-hydroxylation, which is higher than that of 4beta-hydroxylation, a marker activity of CYP3A4
25-hydroxycholesterol + A + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
P05177
gene Ch25h
-
Homo sapiens
P08684
gene Ch25h
-
Homo sapiens
P10635
gene Ch25h
-
Homo sapiens
P11712
gene Ch25h
-
Mus musculus
Q9Z0F5
gene Ch25h
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
AML-12 cell
-
Mus musculus
-
liver
-
Homo sapiens
-
liver
-
Mus musculus
-
additional information
25-hydroxycholesterol concentrations in normal human sera correlates positively with the levels of 4beta-hydroxycholesterol, but does not significantly correlate with the levels of 27-hydroxycholesterol or (24S)-hydroxycholesterol; 25-hydroxycholesterol concentrations in normal human sera correlates positively with the levels of 4beta-hydroxycholesterol, but does not significantly correlate with the levels of 27-hydroxycholesterol or (24S)-hydroxycholesterol; 25-hydroxycholesterol concentrations in normal human sera correlates positively with the levels of 4beta-hydroxycholesterol, but does not significantly correlate with the levels of 27-hydroxycholesterol or (24S)-hydroxycholesterol; 25-hydroxycholesterol concentrations in normal human sera correlates positively with the levels of 4beta-hydroxycholesterol, but does not significantly correlate with the levels of 27-hydroxycholesterol or (24S)-hydroxycholesterol
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cholesterol + AH2 + O2
-
728088
Mus musculus
25-hydroxycholesterol + A + H2O
-
-
-
?
cholesterol + AH2 + O2
-
728088
Homo sapiens
25-hydroxycholesterol + A + H2O
-
-
-
?
cholesterol + AH2 + O2
lower 25-hydroxylation enzyme activity by CYP1A2 compared to marker enzyme CYP3A4
728088
Homo sapiens
25-hydroxycholesterol + A + H2O
-
-
-
?
cholesterol + AH2 + O2
lower 25-hydroxylation enzyme activity by CYP2C9 compared to marker enzyme CYP3A4
728088
Homo sapiens
25-hydroxycholesterol + A + H2O
-
-
-
?
cholesterol + AH2 + O2
lower 25-hydroxylation enzyme activity by CYP2D6 compared to marker enzyme CYP3A4
728088
Homo sapiens
25-hydroxycholesterol + A + H2O
-
-
-
?
cholesterol + AH2 + O2
production of 25-hydroxycholesterol by intact CYP3A4, most significant activity is 25-hydroxylation, which is higher than that of 4beta-hydroxylation, a marker activity of CYP3A4
728088
Homo sapiens
25-hydroxycholesterol + A + H2O
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
recombinant expression in insect cell microsomes via baculovirus transfection
Homo sapiens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
in cells overexpressing human recombinant CYP3A4, the activity of cholesterol 25-hydroxylation is higher than that of cholesterol 4beta-hydroxylation, a known marker activity of CYP3A4
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
desmosterol
a potent inhibitor of CH25H
Homo sapiens
desmosterol
a potent inhibitor of CH25H
Mus musculus
troleandomycin
specific inhibitor of CYP3A
Homo sapiens
troleandomycin
specific inhibitor of CYP3A
Mus musculus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Homo sapiens
-
-
microsome
-
Mus musculus
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
cholesterol + AH2 + O2
Mus musculus
-
25-hydroxycholesterol + A + H2O
-
-
?
cholesterol + AH2 + O2
Homo sapiens
lower 25-hydroxylation enzyme activity by CYP1A2 compared to marker enzyme CYP3A4
25-hydroxycholesterol + A + H2O
-
-
?
cholesterol + AH2 + O2
Homo sapiens
lower 25-hydroxylation enzyme activity by CYP2C9 compared to marker enzyme CYP3A4
25-hydroxycholesterol + A + H2O
-
-
?
cholesterol + AH2 + O2
Homo sapiens
lower 25-hydroxylation enzyme activity by CYP2D6 compared to marker enzyme CYP3A4
25-hydroxycholesterol + A + H2O
-
-
?
cholesterol + AH2 + O2
Homo sapiens
production of 25-hydroxycholesterol by intact CYP3A4, most significant activity is 25-hydroxylation, which is higher than that of 4beta-hydroxylation, a marker activity of CYP3A4
25-hydroxycholesterol + A + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
AML-12 cell
-
Mus musculus
-
liver
-
Homo sapiens
-
liver
-
Mus musculus
-
additional information
25-hydroxycholesterol concentrations in normal human sera correlates positively with the levels of 4beta-hydroxycholesterol, but does not significantly correlate with the levels of 27-hydroxycholesterol or (24S)-hydroxycholesterol
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cholesterol + AH2 + O2
-
728088
Mus musculus
25-hydroxycholesterol + A + H2O
-
-
-
?
cholesterol + AH2 + O2
-
728088
Homo sapiens
25-hydroxycholesterol + A + H2O
-
-
-
?
cholesterol + AH2 + O2
lower 25-hydroxylation enzyme activity by CYP1A2 compared to marker enzyme CYP3A4
728088
Homo sapiens
25-hydroxycholesterol + A + H2O
-
-
-
?
cholesterol + AH2 + O2
lower 25-hydroxylation enzyme activity by CYP2C9 compared to marker enzyme CYP3A4
728088
Homo sapiens
25-hydroxycholesterol + A + H2O
-
-
-
?
cholesterol + AH2 + O2
lower 25-hydroxylation enzyme activity by CYP2D6 compared to marker enzyme CYP3A4
728088
Homo sapiens
25-hydroxycholesterol + A + H2O
-
-
-
?
cholesterol + AH2 + O2
production of 25-hydroxycholesterol by intact CYP3A4, most significant activity is 25-hydroxylation, which is higher than that of 4beta-hydroxylation, a marker activity of CYP3A4
728088
Homo sapiens
25-hydroxycholesterol + A + H2O
-
-
-
?
Expression
Organism
Commentary
Expression
Mus musculus
induction of CYP3A by pregnenolone-16 alpha-carbonitrile causes accumulation of 25-hydroxycholesterol in cell line AML12 derived from mouse liver, the induction is not suppressed by the addition of desmosterol. Although the transcription of Ch25h is also upregulated by addition of pregnenolone-16 alpha-carbonitrile, the protein level of CH25H is not elevated. Desmosterol does not affect the expression of cellular CYP3A protein, but CH25H protein level is obviously decreased by desmosterol treatment
up
Expression (protein specific)
Organism
Commentary
Expression
Mus musculus
induction of CYP3A by pregnenolone-16 alpha-carbonitrile causes accumulation of 25-hydroxycholesterol in cell line AML12 derived from mouse liver, the induction is not suppressed by the addition of desmosterol. Although the transcription of Ch25h is also upregulated by addition of pregnenolone-16 alpha-carbonitrile, the protein level of CH25H is not elevated. Desmosterol does not affect the expression of cellular CYP3A protein, but CH25H protein level is obviously decreased by desmosterol treatment
up
Other publictions for EC 1.14.99.38
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744738
Li
Krueppel-like factor 4 regula ...
Mus musculus
Circulation
136
1315-1330
2017
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745450
Noebauer
Hepatic cholesterol-25-hydrox ...
Mus musculus
J. Diabetes Res.
2017
4108768
2017
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1
1
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745479
Wang
Cholesterol 25-hydroxylase ac ...
Sus scrofa
J. Gen. Virol.
98
1467-1476
2017
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1
1
1
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745518
Mukherjee
Transgenic tomatoes expressin ...
Mus musculus
J. Lipid Res.
58
1636-1647
2017
-
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4
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1
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1
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1
-
-
745658
Ke
Cholesterol 25-hydroxylase in ...
Sus scrofa
J. Virol.
91
e00827-17
2017
-
-
1
-
1
-
-
-
-
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1
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6
-
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2
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1
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1
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2
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1
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1
1
1
1
-
-
745051
Anggakusuma
Interferon-inducible choleste ...
Homo sapiens
Hepatology
62
702-714
2015
-
-
-
-
-
-
-
-
1
-
-
1
-
5
-
-
-
-
-
3
-
-
1
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1
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1
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3
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1
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-
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1
1
1
1
-
-
745657
Xiang
Identification of cholesterol ...
Homo sapiens
J. Virol.
89
6805-6816
2015
-
-
-
-
-
-
-
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-
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1
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5
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2
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1
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1
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2
1
1
2
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746440
Tsujioka
Five-aza-2-deoxycytidine-indu ...
Homo sapiens
Sci. Rep.
5
16709
2015
-
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1
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1
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1
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2
1
1
2
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746435
Chen
Interferon-inducible choleste ...
Homo sapiens
Sci. Rep.
4
7242
2014
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1
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1
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1
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4
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1
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1
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1
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727109
Diczfalusy
On the formation and possible ...
Homo sapiens, Mus musculus, Rattus norvegicus, Sus scrofa
Biochimie
95
455-460
2013
-
-
1
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-
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9
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8
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6
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14
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10
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1
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9
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14
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10
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1
7
7
1
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727611
Liu
Interferon-inducible cholester ...
Mus musculus
Immunity
38
92-105
2013
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1
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1
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7
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1
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2
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726545
Holmes
Genomics and proteomics of ver ...
Bos taurus, Canis lupus familiaris, Equus caballus, Gallus gallus, Homo sapiens, Macaca mulatta, Mus musculus, Rattus norvegicus, Xenopus tropicalis
3 Biotech
1
99-109
2011
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11
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9
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9
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9
9
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728088
Honda
Cholesterol 25-hydroxylation a ...
Homo sapiens, Mus musculus
J. Lipid Res.
52
1509-1516
2011
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1
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1
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4
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1
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1
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715833
Park
Cholesterol 25-hydroxylase pro ...
Mus musculus, Mus musculus BALB/cJ
J. Leukoc. Biol.
88
1081-1087
2010
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1
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5
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1
1
1
1
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699414
Diczfalusy
Marked upregulation of cholest ...
Homo sapiens, Mus musculus
J. Lipid Res.
50
2258-2264
2009
1
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700999
Bauman
25-Hydroxycholesterol secreted ...
Mus musculus
Proc. Natl. Acad. Sci. USA
106
16764-16769
2009
3
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1
1
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2
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12
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3
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1
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Chen
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Russell
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cDNA cloning of mouse and huma ...
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