BRENDA - Enzyme Database
show all sequences of 1.14.99.38

cDNA cloning of mouse and human cholesterol 25-hydroxylases, polytopic membrane proteins that synthesize a potent oxysterol regulator of lipid metabolism

Lund, E.G.; Kerr, T.A.; Sakai, J.; Li, W.P.; Russell, D.W.; J. Biol. Chem. 273, 34316-34327 (1998)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in COS cells, expression of cholesterol 25-hydroxylase in transfected cells reduces the biosynthesis of cholesterol from acetate and suppresses the cleavage of sterol regulatory element binding protein-1 and -2
Homo sapiens
expression in COS cells, expression of cholesterol 25-hydroxylase in transfected cells reduces the biosynthesis of cholesterol from acetate and suppresses the cleavage of sterol regulatory element binding protein-1 and -2
Mus musculus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
polytopic membrane protein
Homo sapiens
16020
-
membrane
polytopic membrane protein
Mus musculus
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe
diiron cofactor
Homo sapiens
Fe
diiron cofactor
Mus musculus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
cholesterol + AH2 + O2
Mus musculus
cholesterol 25-hydroxylase has the capacity to play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein processing and ultimately leads to inhibition of gene transcription
25-hydroxycholesterol + A + H2O
-
-
?
cholesterol + AH2 + O2
Homo sapiens
cholesterol 25-hydroxylase has the capacity to play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein processing and ultimately leads to inhibition of gene transcription
25-hydroxycholesterol + A + H2O
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Homo sapiens
O95992
-
-
Mus musculus
Q9Z0F5
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
cholesterol + AH2 + O2
-
659137
Mus musculus
25-hydroxycholesterol + A + H2O
-
-
-
?
cholesterol + AH2 + O2
-
659137
Homo sapiens
25-hydroxycholesterol + A + H2O
-
-
-
?
cholesterol + AH2 + O2
cholesterol 25-hydroxylase has the capacity to play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein processing and ultimately leads to inhibition of gene transcription
659137
Mus musculus
25-hydroxycholesterol + A + H2O
-
-
-
?
cholesterol + AH2 + O2
cholesterol 25-hydroxylase has the capacity to play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein processing and ultimately leads to inhibition of gene transcription
659137
Homo sapiens
25-hydroxycholesterol + A + H2O
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
cholesterol 25-hydroxylase
-
Homo sapiens
cholesterol 25-hydroxylase
-
Mus musculus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in COS cells, expression of cholesterol 25-hydroxylase in transfected cells reduces the biosynthesis of cholesterol from acetate and suppresses the cleavage of sterol regulatory element binding protein-1 and -2
Homo sapiens
expression in COS cells, expression of cholesterol 25-hydroxylase in transfected cells reduces the biosynthesis of cholesterol from acetate and suppresses the cleavage of sterol regulatory element binding protein-1 and -2
Mus musculus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
polytopic membrane protein
Homo sapiens
16020
-
membrane
polytopic membrane protein
Mus musculus
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe
diiron cofactor
Homo sapiens
Fe
diiron cofactor
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
cholesterol + AH2 + O2
Mus musculus
cholesterol 25-hydroxylase has the capacity to play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein processing and ultimately leads to inhibition of gene transcription
25-hydroxycholesterol + A + H2O
-
-
?
cholesterol + AH2 + O2
Homo sapiens
cholesterol 25-hydroxylase has the capacity to play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein processing and ultimately leads to inhibition of gene transcription
25-hydroxycholesterol + A + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
cholesterol + AH2 + O2
-
659137
Mus musculus
25-hydroxycholesterol + A + H2O
-
-
-
?
cholesterol + AH2 + O2
-
659137
Homo sapiens
25-hydroxycholesterol + A + H2O
-
-
-
?
cholesterol + AH2 + O2
cholesterol 25-hydroxylase has the capacity to play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein processing and ultimately leads to inhibition of gene transcription
659137
Mus musculus
25-hydroxycholesterol + A + H2O
-
-
-
?
cholesterol + AH2 + O2
cholesterol 25-hydroxylase has the capacity to play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein processing and ultimately leads to inhibition of gene transcription
659137
Homo sapiens
25-hydroxycholesterol + A + H2O
-
-
-
?
Other publictions for EC 1.14.99.38
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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136
1315-1330
2017
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1
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1
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1
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-
745450
Noebauer
Hepatic cholesterol-25-hydrox ...
Mus musculus
J. Diabetes Res.
2017
4108768
2017
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1
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3
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1
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1
1
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-
745479
Wang
Cholesterol 25-hydroxylase ac ...
Sus scrofa
J. Gen. Virol.
98
1467-1476
2017
-
-
-
-
1
-
-
-
-
-
-
1
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3
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1
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2
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1
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-
-
-
-
-
-
-
-
1
1
1
1
-
-
745518
Mukherjee
Transgenic tomatoes expressin ...
Mus musculus
J. Lipid Res.
58
1636-1647
2017
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
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-
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4
-
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1
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1
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1
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4
-
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1
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-
-
-
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-
-
1
-
-
1
-
-
745658
Ke
Cholesterol 25-hydroxylase in ...
Sus scrofa
J. Virol.
91
e00827-17
2017
-
-
1
-
1
-
-
-
-
-
-
1
-
6
-
-
-
-
-
2
-
-
1
-
1
-
-
-
-
-
-
-
-
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1
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1
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1
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2
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-
1
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
745051
Anggakusuma
Interferon-inducible choleste ...
Homo sapiens
Hepatology
62
702-714
2015
-
-
-
-
-
-
-
-
1
-
-
1
-
5
-
-
-
-
-
3
-
-
1
-
2
-
-
-
-
-
-
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1
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1
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3
-
-
1
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
745657
Xiang
Identification of cholesterol ...
Homo sapiens
J. Virol.
89
6805-6816
2015
-
-
-
-
-
-
-
-
-
-
-
1
-
5
-
-
-
-
-
2
-
-
1
-
1
-
-
-
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1
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2
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-
1
-
-
-
-
-
-
-
-
-
2
1
1
2
-
-
746440
Tsujioka
Five-aza-2-deoxycytidine-indu ...
Homo sapiens
Sci. Rep.
5
16709
2015
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
4
-
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1
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1
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1
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4
-
-
1
-
-
-
-
-
-
-
-
-
2
1
1
2
-
-
746435
Chen
Interferon-inducible choleste ...
Homo sapiens
Sci. Rep.
4
7242
2014
-
-
1
-
1
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
1
2
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1
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1
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1
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-
1
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
727109
Diczfalusy
On the formation and possible ...
Homo sapiens, Mus musculus, Rattus norvegicus, Sus scrofa
Biochimie
95
455-460
2013
-
-
1
-
-
-
-
-
9
-
-
8
-
6
-
-
-
-
-
14
-
-
10
-
2
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1
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-
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9
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-
8
-
-
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-
14
-
-
10
-
-
-
-
-
-
-
-
-
1
7
7
1
-
-
727611
Liu
Interferon-inducible cholester ...
Mus musculus
Immunity
38
92-105
2013
-
-
1
-
-
-
-
-
-
-
-
1
-
7
-
-
-
-
-
1
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1
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2
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1
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1
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1
2
2
1
-
-
726545
Holmes
Genomics and proteomics of ver ...
Bos taurus, Canis lupus familiaris, Equus caballus, Gallus gallus, Homo sapiens, Macaca mulatta, Mus musculus, Rattus norvegicus, Xenopus tropicalis
3 Biotech
1
99-109
2011
-
-
9
-
-
-
-
-
9
9
9
9
-
11
-
-
-
-
-
9
-
-
9
9
18
-
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-
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9
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-
-
9
-
-
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9
9
9
9
-
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-
-
-
9
-
-
9
9
-
-
-
-
-
-
-
9
-
9
9
-
-
-
728088
Honda
Cholesterol 25-hydroxylation a ...
Homo sapiens, Mus musculus
J. Lipid Res.
52
1509-1516
2011
-
-
1
-
1
-
4
-
2
-
-
5
-
7
-
-
-
-
-
4
-
-
9
-
10
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4
-
-
1
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6
-
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5
-
-
5
-
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-
-
10
-
-
9
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
715833
Park
Cholesterol 25-hydroxylase pro ...
Mus musculus, Mus musculus BALB/cJ
J. Leukoc. Biol.
88
1081-1087
2010
-
-
1
-
-
-
-
-
-
-
-
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3
-
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-
5
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1
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1
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5
-
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-
-
-
-
-
-
-
-
1
1
1
1
-
-
699414
Diczfalusy
Marked upregulation of cholest ...
Homo sapiens, Mus musculus
J. Lipid Res.
50
2258-2264
2009
1
-
-
-
-
-
-
-
-
-
-
2
-
3
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4
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2
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4
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2
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700999
Bauman
25-Hydroxycholesterol secreted ...
Mus musculus
Proc. Natl. Acad. Sci. USA
106
16764-16769
2009
3
-
-
-
-
-
-
-
-
-
1
1
-
2
-
-
-
-
-
12
-
-
1
-
2
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3
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1
1
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12
-
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1
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-
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-
660012
Shibata
Association studies of cholest ...
Homo sapiens
Neurosci. Lett.
391
142-146
2006
-
1
-
-
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-
-
-
-
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1
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1
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1
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1
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-
676167
Papassotiropoulos
Cholesterol 25-hydroxylase on ...
Homo sapiens
Neurodegener. Dis.
2
233-241
2005
-
1
-
-
-
-
-
-
-
-
-
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2
-
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1
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-
658245
Chen
25-hydroxycholesterol is produ ...
Rattus norvegicus
Biol. Reprod.
66
1336-1341
2002
-
-
-
-
-
-
-
-
-
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1
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1
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1
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1
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1
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1
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1
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
658246
Lukyanenko
Testosterone regulates 25-hydr ...
Rattus norvegicus
Biol. Reprod.
67
1435-1438
2002
-
-
-
-
-
-
-
-
-
-
-
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1
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1
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1
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1
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1
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-
-
-
-
-
-
-
-
-
-
-
658244
Lukyanenko
Production of 25-hydroxycholes ...
Rattus norvegicus
Biol. Reprod.
64
790-796
2001
-
-
-
-
-
-
-
-
-
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-
-
-
2
-
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-
1
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1
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