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Literature summary for 1.14.99.29 extracted from

  • Atemnkeng, V.A.; Pink, M.; Schmitz-Spanke, S.; Wu, X.J.; Dong, L.L.; Zhao, K.H.; May, C.; Laufer, S.; Langer, B.; Kaiser, A.
    Deoxyhypusine hydroxylase from Plasmodium vivax, the neglected human malaria parasite: molecular cloning, expression and specific inhibition by the 5-LOX inhibitor zileuton (2013), PLoS ONE, 8, e58318.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.99.29

Cloned(Commentary)

Cloned (Comment) Organism
gene dohh, sequence comparison, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Plasmodium vivax

Inhibitors

Inhibitors Comment Organism Structure
zileuton
-
 Plasmodium vivax

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39010
-
 x * 39010, recombinant His-tagged enzyme, SDS-PAGE Plasmodium vivax

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[eIF5A]-deoxyhypusine + AH2 + O2 Plasmodium vivax
-
 [eIF5A]-hypusine + A + H2O
-
 ?
[eIF5A]-deoxyhypusine + AH2 + O2 Plasmodium vivax Salvador I
-
 [eIF5A]-hypusine + A + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Plasmodium vivax A5JZ19 gene dohh, substrain PEXT-1
-
Plasmodium vivax Salvador I A5JZ19 gene dohh, substrain PEXT-1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affiniity chromatography Plasmodium vivax

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[eIF5A]-deoxyhypusine + AH2 + O2
-
 Plasmodium vivax [eIF5A]-hypusine + A + H2O
-
 ?
[eIF5A]-deoxyhypusine + AH2 + O2
-
 Plasmodium vivax [eIF5A]-hypusine + A + H2O product analysis by GC/MS and gel filtration ?
[eIF5A]-deoxyhypusine + AH2 + O2
-
 Plasmodium vivax Salvador I [eIF5A]-hypusine + A + H2O
-
 ?
[eIF5A]-deoxyhypusine + AH2 + O2
-
 Plasmodium vivax Salvador I [eIF5A]-hypusine + A + H2O product analysis by GC/MS and gel filtration ?

Subunits

Subunits Comment Organism
? x * 39010, recombinant His-tagged enzyme, SDS-PAGE Plasmodium vivax

Synonyms

Synonyms Comment Organism
deoxyhypusine hydroxylase
-
 Plasmodium vivax
DOHH
-
 Plasmodium vivax

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Plasmodium vivax

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Plasmodium vivax

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0000125
-
 recombinant His-tagged enzyme, pH 7.5, 37°C Plasmodium vivax zileuton

General Information

General Information Comment Organism
physiological function deoxyhypusine hydroxylase completes hypusine biosynthesis in eukaryotic initiation factor (eIF-5A) which is the only cellular protein known to contain the unusual amino acid hypusine. Modified EIF-5A is important for proliferation of the malaria parasite Plasmodium vivax