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Literature summary for 1.14.99.29 extracted from

  • Abbruzzese, A.; Park, M.H.; Folk J.E.
    Deoxyhypusine hydroxylase from rat testis. Partial purification and characterization (1986), J. Biol. Chem., 261, 3085-3089.
    View publication on PubMed
Search for more literature for 1.14.99.29

Activating Compound

Activating Compound Comment Organism Structure
sulfhydryl compound absolute requirement of partially purified enzyme, not necessary for crude extract Rattus norvegicus

General Stability

General Stability Organism
20 mM Tris buffer stabilizes Rattus norvegicus
freezing/thawing, stable for numerous times Rattus norvegicus
sulfhydryl compound necessary Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline complete inhibition at 0.01 mM Rattus norvegicus
CaCl2 98% of initial activity at 0.005 mM Rattus norvegicus
Co(C2H3O2)2 94% of initial activity at 0.005 mM Rattus norvegicus
EDTA high ionic strength EDTA, Tris concentrations above 30 mM Rattus norvegicus
FeCl3 65% of initial activity at 0.005 mM Rattus norvegicus
FeSO4 13% of initial activity at 0.005 mM Rattus norvegicus
MnCl2 64% of initial activity at 0.005 mM Rattus norvegicus
additional information no inhibition by CuSO4, HgCl2, MgCl2, CdSO4 at 0.005 mM; partial reconstitution of 0.01 mM 1,10-phenanthroline inhibited activity with Co(C2H3O2)2 at 0.03 mM or FeSO4 at 0.005 mM Rattus norvegicus
NiSO4 72% of initial activity at 0.005 mM Rattus norvegicus
pyridine 2,3-dicarboxylate
-
 Rattus norvegicus
Pyridine 2,4-dicarboxylate
-
 Rattus norvegicus
Pyridine 2,5-dicarboxylate
-
 Rattus norvegicus
Pyridine 3,4-dicarboxylate
-
 Rattus norvegicus
Pyridine 3,5-dicarboxylate
-
 Rattus norvegicus
ZnCl2 93% of initial activity at 0.005 mM Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000052
-
 deoxyhypusine
-
 Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
additional information distribution in mammals Rattus norvegicus
-
testis
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 corresponds to 2.8 pmol/min/mg for partially purified enzyme Rattus norvegicus
416
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, stable for at least 6 months Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N'-(4-aminobutyl)lysine + electron donor + O2 i.e. deoxyhypusine Rattus norvegicus N'-(4-amino-2-hydroxybutyl)lysine + oxidized electron donor + H2O i.e. hypusine ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5 in 20 mM Tris buffer Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADPH, ascorbic acid, mercaptoethylamine, mercaptoacetic acid Rattus norvegicus