Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequenc determination, expression in Escherichia coli strain BL21 | Mycolicibacterium smegmatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
progesterone + AH2 + O2 | Mycolicibacterium smegmatis | reaction with whole cells in a bioreactor | 9alpha-hydroxyprogesterone + A + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli by immobilized metal affinity and ion exchange chromatography | Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-androsten-3,17-dione + AH2 + O2 | - |
Mycolicibacterium smegmatis | ? | - |
? | |
additional information | product determination and identification by LLE, HPLC-DAD, MS, and NMR, overview | Mycolicibacterium smegmatis | ? | - |
? | |
progesterone + AH2 + O2 | reaction with whole cells in a bioreactor | Mycolicibacterium smegmatis | 9alpha-hydroxyprogesterone + A + H2O | - |
? | |
progesterone + AH2 + O2 | reaction with whole cells in a bioreactor, low in vitro activity | Mycolicibacterium smegmatis | 9alpha-hydroxyprogesterone + A + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
steroid 9alpha-hydroxylase | - |
Mycolicibacterium smegmatis |