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Literature summary for 1.14.99.24 extracted from

  • Arnell, R.; Johannisson, R.; Lindholm, J.; Fornstedt, T.; Ersson, B.; Ballagi, A.; Caldwell, K.
    Biotechnological approach to the synthesis of 9alpha-hydroxylated steroids (2007), Prep. Biochem. Biotechnol., 37, 309-321.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequenc determination, expression in Escherichia coli strain BL21 Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
progesterone + AH2 + O2 Mycolicibacterium smegmatis reaction with whole cells in a bioreactor 9alpha-hydroxyprogesterone + A + H2O
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?

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis
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-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli by immobilized metal affinity and ion exchange chromatography Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-androsten-3,17-dione + AH2 + O2
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Mycolicibacterium smegmatis ?
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?
additional information product determination and identification by LLE, HPLC-DAD, MS, and NMR, overview Mycolicibacterium smegmatis ?
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?
progesterone + AH2 + O2 reaction with whole cells in a bioreactor Mycolicibacterium smegmatis 9alpha-hydroxyprogesterone + A + H2O
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?
progesterone + AH2 + O2 reaction with whole cells in a bioreactor, low in vitro activity Mycolicibacterium smegmatis 9alpha-hydroxyprogesterone + A + H2O
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?

Synonyms

Synonyms Comment Organism
steroid 9alpha-hydroxylase
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Mycolicibacterium smegmatis