BRENDA - Enzyme Database show
show all sequences of 1.14.99.24

The inducibility of 9alpha-steroid hydroxylating activity in resting Rhodococcus sp. cells

Mutafov, S.; Angelova, B.; Avramova, T.; Boyadjieva, L.; Dimova, I.; Process Biotechnol. 32, 585-589 (1997)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
ethanol
presence of ethanol enhances the hydroxylation by resting non-induced cells
Rhodococcus sp.
Application
Application
Commentary
Organism
medicine
-
Corynebacterium sp.
medicine
9alpha-steroid hydroxylating activity is used for conversion of different steroid compounds into valuable 9alpha-hydroxy derivatives used in the pharmaceutical industry
Rhodococcus sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4,9(11)-pregnadiene-3,20-dione + NADH + O2
Corynebacterium sp.
-
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
Rhodococcus sp.
key enzyme system controlling the microbial 9alpha-hydroxylation of 4-androstene-3,17-dione
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
Rhodococcus sp. IOC-77
key enzyme system controlling the microbial 9alpha-hydroxylation of 4-androstene-3,17-dione
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Corynebacterium sp.
-
synonym Nocardia restrictus, ATCC 14887 (MCI 1354)
-
Rhodococcus sp.
-
-
-
Rhodococcus sp. IOC-77
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4,9(11)-pregnadiene-3,20-dione + NADH + O2
-
438299
Corynebacterium sp.
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
-
438299
Rhodococcus sp.
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
key enzyme system controlling the microbial 9alpha-hydroxylation of 4-androstene-3,17-dione
438299
Rhodococcus sp.
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
-
438299
Rhodococcus sp. IOC-77
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
key enzyme system controlling the microbial 9alpha-hydroxylation of 4-androstene-3,17-dione
438299
Rhodococcus sp. IOC-77
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
-
?
4-androstene-3,17-dione + NADH + O2
-
438299
Corynebacterium sp.
9alpha-hydroxy-4-androstene-3,17-dione + NAD+ + H2O
-
-
-
?
4-androstene-3,17-dione + NADH + O2
-
438299
Rhodococcus sp.
9alpha-hydroxy-4-androstene-3,17-dione + NAD+ + H2O
-
-
-
?
4-androstene-3,17-dione + NADH + O2
-
438299
Rhodococcus sp. IOC-77
9alpha-hydroxy-4-androstene-3,17-dione + NAD+ + H2O
-
-
-
?
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
ethanol
presence of ethanol enhances the hydroxylation by resting non-induced cells
Rhodococcus sp.
Application (protein specific)
Application
Commentary
Organism
medicine
-
Corynebacterium sp.
medicine
9alpha-steroid hydroxylating activity is used for conversion of different steroid compounds into valuable 9alpha-hydroxy derivatives used in the pharmaceutical industry
Rhodococcus sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4,9(11)-pregnadiene-3,20-dione + NADH + O2
Corynebacterium sp.
-
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
Rhodococcus sp.
key enzyme system controlling the microbial 9alpha-hydroxylation of 4-androstene-3,17-dione
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
Rhodococcus sp. IOC-77
key enzyme system controlling the microbial 9alpha-hydroxylation of 4-androstene-3,17-dione
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4,9(11)-pregnadiene-3,20-dione + NADH + O2
-
438299
Corynebacterium sp.
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
-
438299
Rhodococcus sp.
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
key enzyme system controlling the microbial 9alpha-hydroxylation of 4-androstene-3,17-dione
438299
Rhodococcus sp.
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
-
438299
Rhodococcus sp. IOC-77
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
key enzyme system controlling the microbial 9alpha-hydroxylation of 4-androstene-3,17-dione
438299
Rhodococcus sp. IOC-77
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
-
-
?
4-androstene-3,17-dione + NADH + O2
-
438299
Corynebacterium sp.
9alpha-hydroxy-4-androstene-3,17-dione + NAD+ + H2O
-
-
-
?
4-androstene-3,17-dione + NADH + O2
-
438299
Rhodococcus sp.
9alpha-hydroxy-4-androstene-3,17-dione + NAD+ + H2O
-
-
-
?
4-androstene-3,17-dione + NADH + O2
-
438299
Rhodococcus sp. IOC-77
9alpha-hydroxy-4-androstene-3,17-dione + NAD+ + H2O
-
-
-
?
Other publictions for EC 1.14.99.24
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
713784
Wei
A new steroid-transforming str ...
Mycolicibacterium neoaurum, Mycolicibacterium neoaurum NwIB-01
Appl. Biochem. Biotechnol.
162
1446-1456
2010
-
1
1
-
-
-
-
-
-
-
1
2
-
5
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
1
1
-
-
-
716250
Hu
3-Ketosteroid 9alpha-hydroxyla ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Mol. Microbiol.
75
107-121
2010
-
-
1
-
1
-
-
-
-
-
-
2
-
163
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
3
3
-
-
-
689732
Arnell
Biotechnological approach to t ...
Mycolicibacterium smegmatis
Prep. Biochem. Biotechnol.
37
309-321
2007
-
-
1
-
-
-
-
-
-
-
-
1
-
3
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
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-
1
-
-
-
-
-
-
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-
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-
1
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
438296
Kang
Heterogeneous natures of the m ...
Corynebacterium sp., Mycobacterium sp., Mycolicibacterium fortuitum, Nocardia sp., Nocardia sp. M117, Rhodococcus erythropolis, Rhodococcus erythropolis KCTC 1062, Rhodococcus rhodochrous, Rhodococcus rhodochrous KCTC 1061 / ATCC 17895, Rhodococcus sp.
Arch. Pharm. Res.
20
519-524
1997
-
3
-
-
-
-
16
-
9
-
-
10
-
11
-
-
1
-
-
-
-
-
17
-
-
-
-
-
-
-
-
8
-
-
-
-
3
-
8
-
-
-
-
16
-
-
9
-
-
10
-
-
-
1
-
-
-
-
17
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
438297
Kang
Microbial 9alpha-hydroxylase: ...
Corynebacterium sp.
Arch. Pharm. Res.
20
525-528
1997
-
2
-
-
-
-
-
-
-
-
-
1
-
1
-
-
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-
-
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3
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2
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1
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-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
438298
Kang
Purification of the NADH reduc ...
Mycolicibacterium fortuitum, Nocardia sp.
Arch. Pharm. Res.
20
590-596
1997
-
1
-
-
-
-
-
1
2
2
1
2
-
2
-
-
1
-
-
-
1
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
1
-
4
-
-
-
-
-
-
1
2
2
1
2
-
-
-
1
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
438299
Mutafov
-
The inducibility of 9alpha-ste ...
Corynebacterium sp., Rhodococcus sp., Rhodococcus sp. IOC-77
Process Biotechnol.
32
585-589
1997
1
2
-
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
438295
Sonomoto K.; Usuin N.; Tanaka A.; Fukui S.
-
9alpha-Hydroxylation of 4-andr ...
Corynebacterium sp.
Eur. J. Appl. Microbiol. Biotechnol.
17
203-210
1983
3
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
2
-
-
1
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2
1
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3
-
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-
-
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-
-
1
-
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-
-
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-
-
1
2
-
-
1
-
-
2
1
-
-
-
-
-
-
-
-
438294
Strijewski A.
The steroid-9alpha-hydroxylati ...
Arthrobacter sp., Corynebacterium sp., Mycobacterium sp., Nocardia sp., Nocardia sp. M117
Eur. J. Biochem.
128
125-135
1982
-
4
-
-
-
-
10
-
-
2
2
5
-
6
1
-
1
-
-
-
-
1
5
1
-
-
-
-
1
1
-
4
-
-
-
-
4
-
4
-
-
-
-
10
-
-
-
2
2
5
-
1
-
1
-
-
-
1
5
1
-
-
-
-
1
1
-
-
-
-
-
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-
-