BRENDA - Enzyme Database
show all sequences of 1.14.99.20

Bronsted analysis reveals Lys218 as the carboxylase active site base that deprotonates vitamin K hydroquinone to initiate vitamin K-dependent protein carboxylation

Rishavy, M.A.; Hallgren, K.W.; Yakubenko, A.V.; Shtofman, R.L.; Runge, K.W.; Berkner, K.L.; Biochemistry 45, 13239-13248 (2006)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
H160A
94% of wild-type activity
Leptospira interrogans
H287A
163% of wild-type activity
Leptospira interrogans
H381A
142% of wild-type activity
Leptospira interrogans
K218A
no residual activity, but exogenous amines restore activity and its degree depends on the basicity of the amine
Leptospira interrogans
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Leptospira interrogans
-
-
-
Reaction
Reaction
Commentary
Organism
phylloquinone + reduced acceptor + O2 = 2,3-epoxyphylloquinone + acceptor + H2O
K218 is the essential base that deprotonates AH2 to initiate he reaction
Leptospira interrogans
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
H160A
94% of wild-type activity
Leptospira interrogans
H287A
163% of wild-type activity
Leptospira interrogans
H381A
142% of wild-type activity
Leptospira interrogans
K218A
no residual activity, but exogenous amines restore activity and its degree depends on the basicity of the amine
Leptospira interrogans
Other publictions for EC 1.14.99.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744612
Brunner-Ziegler
Influence of proton pump inhi ...
Homo sapiens
Br. J. Haematol.
167
547-553
2014
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672089
Rishavy
Bronsted analysis reveals Lys2 ...
Leptospira interrogans
Biochemistry
45
13239-13248
2006
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4
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674485
Rishavy
The vitamin K-dependent carbox ...
Leptospira interrogans
J. Biol. Chem.
280
34870-34877
2005
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438311
Itoh
Developmental changes of vitam ...
Homo sapiens
Early Hum. Dev.
57
15-23
2000
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438310
Sugiura
Propeptide and glutamate-conta ...
Bos taurus
Proc. Natl. Acad. Sci. USA
94
9069-9074
1997
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438309
Sugiura
Profactor IX propeptide and gl ...
Bos taurus
J. Biol. Chem.
271
17837-17844
1996
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438308
Roth
Mutagenesis of vitamin K-depen ...
Bos taurus
J. Biol. Chem.
270
5305-5311
1995
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438307
Hubbard
Vitamin K-dependent carboxylas ...
Bos taurus
Proc. Natl. Acad. Sci. USA
86
6893-6897
1989
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438306
McTigue
Oxygen dependence of vitamin K ...
Rattus norvegicus
FEBS Lett.
200
71-75
1986
2
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438302
DeMetz
The inhibition of vitamin K-de ...
Bos taurus
FEBS Lett.
137
253-256
1982
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438305
Wallin
Vitamin K-dependent carboxylas ...
Rattus norvegicus
Arch. Biochem. Biophys.
214
155-163
1982
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438301
Suttie
Vitamin K epoxidase: dependenc ...
Rattus norvegicus
FEBS Lett.
109
267-270
1980
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438303
Larson
Vitamin K-dependent carboxylas ...
Rattus norvegicus
Proc. Natl. Acad. Sci. USA
75
5413-5416
1978
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438304
Sadowski
Vitamin K epoxidase: propertie ...
Rattus norvegicus
Biochemistry
16
3856-3863
1977
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438300
Willingham
Changes in phylloquinone epoxi ...
Rattus norvegicus
Biochem. J.
140
435-441
1974
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