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Literature summary for 1.14.99.20 extracted from

  • Sugiura, I.; Furie, B.; Walsh, C.T.; Furie, B.C.
    Profactor IX propeptide and glutamate substrate binding sites on the vitamin K-dependent carboxylase identified by site-directed mutagenesis (1996), J. Biol. Chem., 271, 17837-17844.
    View publication on PubMed
Search for more literature for 1.14.99.20

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutants of bovine enzyme species expressed in chinese hamster ovary cells CHO-Dukx-B11 Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phylloquinone + electron donor + O2 Bos taurus
-
 2,3-epoxyphylloquinone + oxidized electron + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
phylloquinone + reduced acceptor + O2 = 2,3-epoxyphylloquinone + acceptor + H2O enzyme has two distinct functions: vitamin K epoxidation and gamma-glutamyl carboxylation Bos taurus
a

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bos taurus

Storage Stability

Storage Stability Organism
-80°C, 20% glycerol Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phylloquinone + electron donor + O2
-
 Bos taurus 2,3-epoxyphylloquinone + oxidized electron donor + H2O
-
 ?
phylloquinone + electron donor + O2
-
 Bos taurus 2,3-epoxyphylloquinone + oxidized electron + H2O
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 enzyme assay Bos taurus