BRENDA - Enzyme Database
show all sequences of 1.14.20.7

Biochemical and spectroscopic characterization of the non-heme Fe(II)- and 2-oxoglutarate-dependent ethylene-forming enzyme from Pseudomonas syringae pv. phaseolicola PK2

Martinez, S.; Hausinger, R.P.; Biochemistry 55, 5989-5999 (2016)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
ascorbate
required
Pseudomonas savastanoi pv. phaseolicola
ascorbate
1 mM used in assay conditions
Pseudomonas syringae
Cloned(Commentary)
Commentary
Organism
gene efe, large scale expression of His6-tagged enzyme in Escherichia coli strain BL21 Gold (DE3), method optimization and evaluation
Pseudomonas savastanoi pv. phaseolicola
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.031
-
2-oxoadipate
pH 7.5, 25C, recombinant enzyme
Pseudomonas savastanoi pv. phaseolicola
0.031
-
2-oxoadipate
at pH 7.5 and 25C
Pseudomonas syringae
0.05
-
L-arginine
with 2-oxoglutarate as cosubstrate, at pH 7.5 and 25C
Pseudomonas syringae
0.057
-
2-oxoglutarate
pH 7.5, 25C, recombinant enzyme
Pseudomonas savastanoi pv. phaseolicola
0.057
-
2-oxoglutarate
at pH 7.5 and 25C
Pseudomonas syringae
0.071
-
L-arginine
with 2-oxoadipate as cosubstrate, at pH 7.5 and 25C
Pseudomonas syringae
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
dependent on, required for catalysis
Pseudomonas savastanoi pv. phaseolicola
Fe2+
required
Pseudomonas syringae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39370
-
recombinant detagged enzyme, gel filtration
Pseudomonas savastanoi pv. phaseolicola
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-oxoglutarate + L-arginine + O2
Pseudomonas syringae
-
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
?
2-oxoglutarate + L-arginine + O2
Pseudomonas savastanoi pv. phaseolicola
-
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
?
2-oxoglutarate + L-arginine + O2
Pseudomonas savastanoi pv. phaseolicola PK2
-
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
?
2-oxoglutarate + L-arginine + O2
Pseudomonas syringae PK2
-
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas savastanoi pv. phaseolicola
P32021
i.e. Kudzu strain
-
Pseudomonas savastanoi pv. phaseolicola PK2
P32021
i.e. Kudzu strain
-
Pseudomonas syringae
-
pv. phaseolicola
-
Pseudomonas syringae PK2
-
pv. phaseolicola
-
Purification (Commentary)
Commentary
Organism
Ni-NTA agarose column chromatography
Pseudomonas syringae
recombinant His-tagged enzyme from Escherichia coli strain BL21 Gold (DE3) by nickel affinity chromatography, tag cleavage by a TEV protease mutant, and another step of nickel affinity chromatography, and dialysis of the flow through
Pseudomonas savastanoi pv. phaseolicola
Reaction
Reaction
Commentary
Organism
L-arginine + 2-oxoglutarate + O2 = succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
reaction mechanism
Pseudomonas savastanoi pv. phaseolicola
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxoadipate + L-arginine + O2
-
741936
Pseudomonas syringae
?
-
-
-
?
2-oxoadipate + L-arginine + O2
-
741936
Pseudomonas syringae PK2
?
-
-
-
?
2-oxoadipate + L-arginine + O2
-
741936
Pseudomonas savastanoi pv. phaseolicola
glutarate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
-
?
2-oxoadipate + L-arginine + O2
-
741936
Pseudomonas savastanoi pv. phaseolicola PK2
glutarate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
-
?
2-oxoglutarate + agmatine + O2
less than 1% activity compared to L-arginine
741936
Pseudomonas syringae
?
-
-
-
?
2-oxoglutarate + agmatine + O2
less than 1% activity compared to L-arginine
741936
Pseudomonas syringae PK2
?
-
-
-
?
2-oxoglutarate + L-argininamide + O2
less than 1% activity compared to L-arginine
741936
Pseudomonas syringae
?
-
-
-
?
2-oxoglutarate + L-arginine + O2
-
741936
Pseudomonas syringae
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
-
?
2-oxoglutarate + L-arginine + O2
-
741936
Pseudomonas savastanoi pv. phaseolicola
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
-
?
2-oxoglutarate + L-arginine + O2
100% activity
741936
Pseudomonas syringae
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
-
?
2-oxoglutarate + L-arginine + O2
-
741936
Pseudomonas savastanoi pv. phaseolicola PK2
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
-
?
2-oxoglutarate + L-arginine + O2
-
741936
Pseudomonas syringae PK2
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
-
?
2-oxoglutarate + L-arginine + O2
100% activity
741936
Pseudomonas syringae PK2
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
-
?
2-oxoglutarate + L-canavanine + O2
less than 1% activity compared to L-arginine
741936
Pseudomonas syringae
?
-
-
-
?
2-oxoglutarate + L-homoarginine + O2
less than 1% activity compared to L-arginine
741936
Pseudomonas syringae
?
-
-
-
?
2-oxoglutarate + L-ornithine + O2
less than 1% activity compared to L-arginine
741936
Pseudomonas syringae
?
-
-
-
?
2-oxoglutarate + Ngamma-hydroxy-L-arginine + O2
15% activity compared to L-arginine
741936
Pseudomonas syringae
?
-
-
-
?
additional information
no activity with 2-oxovalerate, 2-oxobutyrate, pyruvate, and oxalate
741936
Pseudomonas syringae
?
-
-
-
-
additional information
cf. EC 1.13.12.19, ethylene production. Selected L-Arg derivatives induce ethylene formation without undergoing hydroxylation, demonstrating that ethylen production and L-Arg hydroxylation activities are not linked. Enzyme EFE utilizes the alternative 2-oxo acid 2-oxoadipate as a cosubstrate (forming glutaric acid) during the hydroxylation of L-Arg, with this reaction unlinked from ethylene formation. The amount of ethylene produced is more than twice the levels of succinate, L-DELTA1-pyrroline-5-carboxylate, or guanidine generated
741936
Pseudomonas savastanoi pv. phaseolicola
?
-
-
-
-
additional information
cf. EC 1.13.12.19, ethylene production. Selected L-Arg derivatives induce ethylene formation without undergoing hydroxylation, demonstrating that ethylen production and L-Arg hydroxylation activities are not linked. Enzyme EFE utilizes the alternative 2-oxo acid 2-oxoadipate as a cosubstrate (forming glutaric acid) during the hydroxylation of L-Arg, with this reaction unlinked from ethylene formation. The amount of ethylene produced is more than twice the levels of succinate, L-DELTA1-pyrroline-5-carboxylate, or guanidine generated
741936
Pseudomonas savastanoi pv. phaseolicola PK2
?
-
-
-
-
additional information
no activity with 2-oxovalerate, 2-oxobutyrate, pyruvate, and oxalate
741936
Pseudomonas syringae PK2
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 40000, recombinant detagged enzyme, SDS-PAGE, 1 * 39670, recombinant detagged enzyme, mass spectrometry
Pseudomonas savastanoi pv. phaseolicola
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Pseudomonas savastanoi pv. phaseolicola
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0042
-
2-oxoadipate
pH 7.5, 25C, recombinant enzyme
Pseudomonas savastanoi pv. phaseolicola
0.0042
-
2-oxoadipate
at pH 7.5 and 25C
Pseudomonas syringae
0.0045
-
L-arginine
with 2-oxoadipate as cosubstrate, at pH 7.5 and 25C
Pseudomonas syringae
0.048
-
L-arginine
at pH 7.5 and 25C
Pseudomonas syringae
2.067
-
2-oxoglutarate
pH 7.5, 25C, recombinant enzyme
Pseudomonas savastanoi pv. phaseolicola
2.07
-
2-oxoglutarate
at pH 7.5 and 25C
Pseudomonas syringae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
7.5
broad optimum
Pseudomonas savastanoi pv. phaseolicola
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
9
over 50% of maximal activity within this range
Pseudomonas savastanoi pv. phaseolicola
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
ascorbate
required
Pseudomonas savastanoi pv. phaseolicola
ascorbate
1 mM used in assay conditions
Pseudomonas syringae
Cloned(Commentary) (protein specific)
Commentary
Organism
gene efe, large scale expression of His6-tagged enzyme in Escherichia coli strain BL21 Gold (DE3), method optimization and evaluation
Pseudomonas savastanoi pv. phaseolicola
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.031
-
2-oxoadipate
pH 7.5, 25C, recombinant enzyme
Pseudomonas savastanoi pv. phaseolicola
0.031
-
2-oxoadipate
at pH 7.5 and 25C
Pseudomonas syringae
0.05
-
L-arginine
with 2-oxoglutarate as cosubstrate, at pH 7.5 and 25C
Pseudomonas syringae
0.057
-
2-oxoglutarate
pH 7.5, 25C, recombinant enzyme
Pseudomonas savastanoi pv. phaseolicola
0.057
-
2-oxoglutarate
at pH 7.5 and 25C
Pseudomonas syringae
0.071
-
L-arginine
with 2-oxoadipate as cosubstrate, at pH 7.5 and 25C
Pseudomonas syringae
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
dependent on, required for catalysis
Pseudomonas savastanoi pv. phaseolicola
Fe2+
required
Pseudomonas syringae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39370
-
recombinant detagged enzyme, gel filtration
Pseudomonas savastanoi pv. phaseolicola
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-oxoglutarate + L-arginine + O2
Pseudomonas syringae
-
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
?
2-oxoglutarate + L-arginine + O2
Pseudomonas savastanoi pv. phaseolicola
-
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
?
2-oxoglutarate + L-arginine + O2
Pseudomonas savastanoi pv. phaseolicola PK2
-
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
?
2-oxoglutarate + L-arginine + O2
Pseudomonas syringae PK2
-
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA agarose column chromatography
Pseudomonas syringae
recombinant His-tagged enzyme from Escherichia coli strain BL21 Gold (DE3) by nickel affinity chromatography, tag cleavage by a TEV protease mutant, and another step of nickel affinity chromatography, and dialysis of the flow through
Pseudomonas savastanoi pv. phaseolicola
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxoadipate + L-arginine + O2
-
741936
Pseudomonas syringae
?
-
-
-
?
2-oxoadipate + L-arginine + O2
-
741936
Pseudomonas syringae PK2
?
-
-
-
?
2-oxoadipate + L-arginine + O2
-
741936
Pseudomonas savastanoi pv. phaseolicola
glutarate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
-
?
2-oxoadipate + L-arginine + O2
-
741936
Pseudomonas savastanoi pv. phaseolicola PK2
glutarate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
-
?
2-oxoglutarate + agmatine + O2
less than 1% activity compared to L-arginine
741936
Pseudomonas syringae
?
-
-
-
?
2-oxoglutarate + agmatine + O2
less than 1% activity compared to L-arginine
741936
Pseudomonas syringae PK2
?
-
-
-
?
2-oxoglutarate + L-argininamide + O2
less than 1% activity compared to L-arginine
741936
Pseudomonas syringae
?
-
-
-
?
2-oxoglutarate + L-arginine + O2
-
741936
Pseudomonas syringae
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
-
?
2-oxoglutarate + L-arginine + O2
-
741936
Pseudomonas savastanoi pv. phaseolicola
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
-
?
2-oxoglutarate + L-arginine + O2
100% activity
741936
Pseudomonas syringae
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
-
?
2-oxoglutarate + L-arginine + O2
-
741936
Pseudomonas savastanoi pv. phaseolicola PK2
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
-
?
2-oxoglutarate + L-arginine + O2
-
741936
Pseudomonas syringae PK2
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
-
?
2-oxoglutarate + L-arginine + O2
100% activity
741936
Pseudomonas syringae PK2
succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
-
-
?
2-oxoglutarate + L-canavanine + O2
less than 1% activity compared to L-arginine
741936
Pseudomonas syringae
?
-
-
-
?
2-oxoglutarate + L-homoarginine + O2
less than 1% activity compared to L-arginine
741936
Pseudomonas syringae
?
-
-
-
?
2-oxoglutarate + L-ornithine + O2
less than 1% activity compared to L-arginine
741936
Pseudomonas syringae
?
-
-
-
?
2-oxoglutarate + Ngamma-hydroxy-L-arginine + O2
15% activity compared to L-arginine
741936
Pseudomonas syringae
?
-
-
-
?
additional information
no activity with 2-oxovalerate, 2-oxobutyrate, pyruvate, and oxalate
741936
Pseudomonas syringae
?
-
-
-
-
additional information
cf. EC 1.13.12.19, ethylene production. Selected L-Arg derivatives induce ethylene formation without undergoing hydroxylation, demonstrating that ethylen production and L-Arg hydroxylation activities are not linked. Enzyme EFE utilizes the alternative 2-oxo acid 2-oxoadipate as a cosubstrate (forming glutaric acid) during the hydroxylation of L-Arg, with this reaction unlinked from ethylene formation. The amount of ethylene produced is more than twice the levels of succinate, L-DELTA1-pyrroline-5-carboxylate, or guanidine generated
741936
Pseudomonas savastanoi pv. phaseolicola
?
-
-
-
-
additional information
cf. EC 1.13.12.19, ethylene production. Selected L-Arg derivatives induce ethylene formation without undergoing hydroxylation, demonstrating that ethylen production and L-Arg hydroxylation activities are not linked. Enzyme EFE utilizes the alternative 2-oxo acid 2-oxoadipate as a cosubstrate (forming glutaric acid) during the hydroxylation of L-Arg, with this reaction unlinked from ethylene formation. The amount of ethylene produced is more than twice the levels of succinate, L-DELTA1-pyrroline-5-carboxylate, or guanidine generated
741936
Pseudomonas savastanoi pv. phaseolicola PK2
?
-
-
-
-
additional information
no activity with 2-oxovalerate, 2-oxobutyrate, pyruvate, and oxalate
741936
Pseudomonas syringae PK2
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 40000, recombinant detagged enzyme, SDS-PAGE, 1 * 39670, recombinant detagged enzyme, mass spectrometry
Pseudomonas savastanoi pv. phaseolicola
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Pseudomonas savastanoi pv. phaseolicola
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0042
-
2-oxoadipate
pH 7.5, 25C, recombinant enzyme
Pseudomonas savastanoi pv. phaseolicola
0.0042
-
2-oxoadipate
at pH 7.5 and 25C
Pseudomonas syringae
0.0045
-
L-arginine
with 2-oxoadipate as cosubstrate, at pH 7.5 and 25C
Pseudomonas syringae
0.048
-
L-arginine
at pH 7.5 and 25C
Pseudomonas syringae
2.067
-
2-oxoglutarate
pH 7.5, 25C, recombinant enzyme
Pseudomonas savastanoi pv. phaseolicola
2.07
-
2-oxoglutarate
at pH 7.5 and 25C
Pseudomonas syringae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
7.5
broad optimum
Pseudomonas savastanoi pv. phaseolicola
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
9
over 50% of maximal activity within this range
Pseudomonas savastanoi pv. phaseolicola
General Information
General Information
Commentary
Organism
evolution
ethylene-forming enzyme (EFE) is a member of the mononuclear non-heme Fe(II)- and 2-oxoglutarate (2OG)-dependent oxygenase superfamily
Pseudomonas savastanoi pv. phaseolicola
physiological function
the enzyme is reported to simultaneously catalyze the conversion of 2OG into ethylene plus three CO2 and the Cdelta hydroxylation of L-arginine (L-Arg) while oxidatively decarboxylating 2-oxoglutarate to form succinate and carbon dioxide. The enzyme produces ethylene, a gas that is widely used as a building block in the production of various plastics, detergents, surfactants, antifreeze, solvents, and other important industrial materials. And ethylene is a plant hormone that plays an important role in growth and development. The ethylene-forming reaction is not intrinsically linked to L-Arg hydroxylation
Pseudomonas savastanoi pv. phaseolicola
General Information (protein specific)
General Information
Commentary
Organism
evolution
ethylene-forming enzyme (EFE) is a member of the mononuclear non-heme Fe(II)- and 2-oxoglutarate (2OG)-dependent oxygenase superfamily
Pseudomonas savastanoi pv. phaseolicola
physiological function
the enzyme is reported to simultaneously catalyze the conversion of 2OG into ethylene plus three CO2 and the Cdelta hydroxylation of L-arginine (L-Arg) while oxidatively decarboxylating 2-oxoglutarate to form succinate and carbon dioxide. The enzyme produces ethylene, a gas that is widely used as a building block in the production of various plastics, detergents, surfactants, antifreeze, solvents, and other important industrial materials. And ethylene is a plant hormone that plays an important role in growth and development. The ethylene-forming reaction is not intrinsically linked to L-Arg hydroxylation
Pseudomonas savastanoi pv. phaseolicola
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.134
-
2-oxoadipate
pH 7.5, 25C, recombinant enzyme
Pseudomonas savastanoi pv. phaseolicola
0.97
-
L-arginine
at pH 7.5 and 25C
Pseudomonas syringae
36.26
-
2-oxoglutarate
pH 7.5, 25C, recombinant enzyme
Pseudomonas savastanoi pv. phaseolicola
36.33
-
2-oxoglutarate
at pH 7.5 and 25C
Pseudomonas syringae
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.134
-
2-oxoadipate
pH 7.5, 25C, recombinant enzyme
Pseudomonas savastanoi pv. phaseolicola
0.97
-
L-arginine
at pH 7.5 and 25C
Pseudomonas syringae
36.26
-
2-oxoglutarate
pH 7.5, 25C, recombinant enzyme
Pseudomonas savastanoi pv. phaseolicola
36.33
-
2-oxoglutarate
at pH 7.5 and 25C
Pseudomonas syringae
Other publictions for EC 1.14.20.7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742750
Martinez
Structures and mechanisms of ...
Pseudomonas savastanoi pv. phaseolicola, Pseudomonas savastanoi pv. phaseolicola PK2
J. Am. Chem. Soc.
139
11980-11988
2017
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6
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1
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1
32
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6
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1
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