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Literature summary for 1.14.20.7 extracted from

  • Guerrero, F.; Carbonell, V.; Cossu, M.; Correddu, D.; Jones, P.R.
    Ethylene synthesis and regulated expression of recombinant protein in Synechocystis sp. PCC 6803 (2012), PLoS ONE, 7, e50470.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene efe, codon optimization, gene synthesis, and construction of the self-replicating wide-host-range pDF-series vectors, recombinant expression in Synechocystis sp. strain PCC 6803, the cyanobacterial system is highly unstable resulting in rapid development of mutants that lost the capability to synthesize ethylene, N-terminally His6-tagged enzyme expression in Escherichia coli. Ethylene synthesis in Escherichia coli and Synechocystis using three different metal-inducible promoters from cyanobacteria, expression rates and genomic structure of the native metal-inducible promoter elements, method development, overview Pseudomonas syringae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-oxoglutarate + L-arginine + O2 Pseudomonas syringae
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succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
?
additional information Pseudomonas syringae in a few plant pathogens ethylene is synthesized by an ethylene forming enzyme in a complex multi-step reaction utilizing 2-oxoglutarate, arginine and dioxygen as substrates, resulting in the accumulation of ethylene in the headspace of closed vessels ?
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?

Organism

Organism UniProt Comment Textmining
Pseudomonas syringae
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pv. phaseolicola
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Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography Pseudomonas syringae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + L-arginine + O2
-
Pseudomonas syringae succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
?
additional information in a few plant pathogens ethylene is synthesized by an ethylene forming enzyme in a complex multi-step reaction utilizing 2-oxoglutarate, arginine and dioxygen as substrates, resulting in the accumulation of ethylene in the headspace of closed vessels Pseudomonas syringae ?
-
?

Synonyms

Synonyms Comment Organism
2-oxoglutarate-dependent ethylene/succinate-forming enzyme
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Pseudomonas syringae
EFE
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Pseudomonas syringae
ethylene forming enzyme
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Pseudomonas syringae