Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Plagiochasma appendiculatum |
Protein Variants | Comment | Organism |
---|---|---|
A120M | the mutant enzyme is able to oxidize dihydrokaempferol to kaempferol with 162% of the catalytic activity relative to wild-type enzyme | Plagiochasma appendiculatum |
F146I | the mutant enzyme is able to oxidize dihydrokaempferol to kaempferol with 86% of the catalytic activity relative to wild-type enzyme | Plagiochasma appendiculatum |
L311F | the mutant enzyme is able to oxidize dihydrokaempferol to kaempferol with 355% of the catalytic activity relative to wild-type enzyme | Plagiochasma appendiculatum |
Y240P | the mutant enzyme is able to oxidize dihydrokaempferol to kaempferol with 104% of the catalytic activity relative to wild-type enzyme | Plagiochasma appendiculatum |
Y240P/A120M | the mutant enzyme is able to oxidize dihydrokaempferol to kaempferol with 175% of the catalytic activity relative to wild-type enzyme | Plagiochasma appendiculatum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plagiochasma appendiculatum | A0A076U8J8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Plagiochasma appendiculatum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrokaempferol + 2-oxoglutarate + O2 | the mutated enzymes L311F, Y240P, F146I, A120M and L311F-A120M are all able to oxidize dihydrokaempferol to kaempferol, with respective catalytic activities relative to the wild type level of 355%, 104%, 86%, 162% and 175%. L311F substitution increases the flavonol synthase activity | Plagiochasma appendiculatum | kaempferol + succinate + CO2 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 61000, SDS-PAGE | Plagiochasma appendiculatum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Plagiochasma appendiculatum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Plagiochasma appendiculatum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Plagiochasma appendiculatum | calculated from sequence | - |
6.42 |
General Information | Comment | Organism |
---|---|---|
physiological function | involved in flavonoid pathway | Plagiochasma appendiculatum |