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Literature summary for 1.14.20.6 extracted from

  • Han, X.J.; Wu, Y.F.; Gao, S.; Yu, H.N.; Xu, R.X.; Lou, H.X.; Cheng, A.X.
    Functional characterization of a Plagiochasma appendiculatum flavone synthase I showing flavanone 2-hydroxylase activity (2014), FEBS Lett., 588, 2307-2314 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Plagiochasma appendiculatum

Protein Variants

Protein Variants Comment Organism
A120M the mutant enzyme is able to oxidize dihydrokaempferol to kaempferol with 162% of the catalytic activity relative to wild-type enzyme Plagiochasma appendiculatum
F146I the mutant enzyme is able to oxidize dihydrokaempferol to kaempferol with 86% of the catalytic activity relative to wild-type enzyme Plagiochasma appendiculatum
L311F the mutant enzyme is able to oxidize dihydrokaempferol to kaempferol with 355% of the catalytic activity relative to wild-type enzyme Plagiochasma appendiculatum
Y240P the mutant enzyme is able to oxidize dihydrokaempferol to kaempferol with 104% of the catalytic activity relative to wild-type enzyme Plagiochasma appendiculatum
Y240P/A120M the mutant enzyme is able to oxidize dihydrokaempferol to kaempferol with 175% of the catalytic activity relative to wild-type enzyme Plagiochasma appendiculatum

Organism

Organism UniProt Comment Textmining
Plagiochasma appendiculatum A0A076U8J8
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Plagiochasma appendiculatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydrokaempferol + 2-oxoglutarate + O2 the mutated enzymes L311F, Y240P, F146I, A120M and L311F-A120M are all able to oxidize dihydrokaempferol to kaempferol, with respective catalytic activities relative to the wild type level of 355%, 104%, 86%, 162% and 175%. L311F substitution increases the flavonol synthase activity Plagiochasma appendiculatum kaempferol + succinate + CO2 + H2O
-
?

Subunits

Subunits Comment Organism
? x * 61000, SDS-PAGE Plagiochasma appendiculatum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Plagiochasma appendiculatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Plagiochasma appendiculatum

pI Value

Organism Comment pI Value Maximum pI Value
Plagiochasma appendiculatum calculated from sequence
-
6.42

General Information

General Information Comment Organism
physiological function involved in flavonoid pathway Plagiochasma appendiculatum