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Literature summary for 1.14.20.6 extracted from
- Isolation, characterization, and function analysis of a flavonol synthase gene from Ginkgo biloba (2012), Mol. Biol. Rep., 39, 2285-2296.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene GbFLS, DNA and amino acid sequence determination and analysis, promoter region sequence analysis and putative cis-acting regulatory elements, overview, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Ginkgo biloba |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | dependent on | Ginkgo biloba |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2R/3R)-dihydrokaempferol + 2-oxoglutarate + O2 | Ginkgo biloba | - |
kaempferol + succinate + CO2 + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ginkgo biloba | D0UZK7 | gene GbFLS | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by immunoaffinity chromatography | Ginkgo biloba |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2R/3R)-dihydrokaempferol + 2-oxoglutarate + O2 | - |
Ginkgo biloba | kaempferol + succinate + CO2 + H2O | - |
? | |
| naringenin + 2-oxoglutarate + O2 | - |
Ginkgo biloba | kaempferol + succinate + CO2 + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FLS | - |
Ginkgo biloba |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Ginkgo biloba |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Ginkgo biloba |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Ginkgo biloba | GbFLS expression is induced by abiotic stresses: UV-B irradiation, abscisic acid, cold, sucrose, salicylic acid, and ethephon | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the 2-oxoglutarate iron-dependent oxygenase family | Ginkgo biloba |
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Release 2023.1 (January 2023)
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