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Literature summary for 1.14.20.6 extracted from

  • Kim, B.; Joe, E.; Ahn, J.
    Molecular characterization of flavonol synthase from poplar and its application to the synthesis of 3-O-methylkaempferol (2010), Biotechnol. Lett., 32, 579-584.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-oxoglutarate activity is completely abolished by the omission of 2-oxoglutarate from the reaction mixture Populus deltoides
ascorbate no reaction product is generated in the absence of ascorbate Populus deltoides
catalase without the addition of catalase, the enzyme activity is decreased to approximately 45% Populus deltoides

Cloned(Commentary)

Cloned (Comment) Organism
expressed with a glutathione-S-transferase tagging in Escherichia coli Populus deltoides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
(2R/3R)-dihydrokaempferol in 10 mM Tris/HCl buffer (pH 8.0), at 37°C Populus deltoides
0.031
-
(2R/3R)-dihydroquercetin in 10 mM Tris/HCl buffer (pH 8.0), at 37°C Populus deltoides

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ dependent on, the omission of Fe2+ in the reaction mixture results in 65% loss of enzyme activity Populus deltoides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
x * 38000, SDS-PAGE Populus deltoides

Organism

Organism UniProt Comment Textmining
Populus deltoides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Populus deltoides

Storage Stability

Storage Stability Organism
4°C, purified enzyme, 2 weeks, no loss of catalytic activity Populus deltoides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R/3R)-dihydrokaempferol + 2-oxoglutarate + O2
-
Populus deltoides kaempferol + succinate + CO2 + H2O
-
?
(2R/3R)-dihydroquercetin + 2-oxoglutarate + O2
-
Populus deltoides quercetin + succinate + CO2 + H2O
-
?
naringenin + 2-oxoglutarate + O2
-
Populus deltoides kaempferol + succinate + CO2 + H2O in a second reaction step with kaempferol 3-O-methyltransferase kaempferol is converted into 3-O-methylkaempferol ?

Subunits

Subunits Comment Organism
? x * 38000, SDS-PAGE Populus deltoides

Synonyms

Synonyms Comment Organism
flavonol synthase
-
Populus deltoides
FLS
-
Populus deltoides