Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | activity is completely abolished by the omission of 2-oxoglutarate from the reaction mixture | Populus deltoides | |
ascorbate | no reaction product is generated in the absence of ascorbate | Populus deltoides | |
catalase | without the addition of catalase, the enzyme activity is decreased to approximately 45% | Populus deltoides |
Cloned (Comment) | Organism |
---|---|
expressed with a glutathione-S-transferase tagging in Escherichia coli | Populus deltoides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
(2R/3R)-dihydrokaempferol | in 10 mM Tris/HCl buffer (pH 8.0), at 37°C | Populus deltoides | |
0.031 | - |
(2R/3R)-dihydroquercetin | in 10 mM Tris/HCl buffer (pH 8.0), at 37°C | Populus deltoides |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | dependent on, the omission of Fe2+ in the reaction mixture results in 65% loss of enzyme activity | Populus deltoides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
x * 38000, SDS-PAGE | Populus deltoides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus deltoides | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Populus deltoides |
Storage Stability | Organism |
---|---|
4°C, purified enzyme, 2 weeks, no loss of catalytic activity | Populus deltoides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R/3R)-dihydrokaempferol + 2-oxoglutarate + O2 | - |
Populus deltoides | kaempferol + succinate + CO2 + H2O | - |
? | |
(2R/3R)-dihydroquercetin + 2-oxoglutarate + O2 | - |
Populus deltoides | quercetin + succinate + CO2 + H2O | - |
? | |
naringenin + 2-oxoglutarate + O2 | - |
Populus deltoides | kaempferol + succinate + CO2 + H2O | in a second reaction step with kaempferol 3-O-methyltransferase kaempferol is converted into 3-O-methylkaempferol | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, SDS-PAGE | Populus deltoides |
Synonyms | Comment | Organism |
---|---|---|
flavonol synthase | - |
Populus deltoides |
FLS | - |
Populus deltoides |