BRENDA - Enzyme Database
show all sequences of 1.14.20.5

Analysis of flavonoids and characterization of the OsFNS gene involved in flavone biosynthesis in rice

Jeong, H.; Young, M.; Kim, B.; Ahn, J.; J. Plant Biol. 51, 97-101 (2008)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
oxoglutarate
no flavone synthase activity without this compound
Oryza sativa
Cloned(Commentary)
Cloned (Commentary)
Organism
RT-PCR cloning of flavone synthase cDNA, subcloning in pGEX 5X-2 for expression in Escherichia coli
Oryza sativa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
S-naringenin
37C, 50 microM FeSO4, 100 mg/ml catalase, 160 microM oxoglutarate, 1 mM ascorbate, 60 microM substrate in 10 mM Tris/HCl buffer, pH 8.0
Oryza sativa
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
FeSO4
65% reduced activity without FeSO4
Oryza sativa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38700
-
SDS-PAGE, 1027 bp, Fe2+-binding site, oxoglutarate binding site
Oryza sativa
64700
-
SDS-PAGE, flavone synthase + glutathione S-transferase (26000 Da)
Oryza sativa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
S-naringenin + 2-oxoglutarate + O2
Oryza sativa
purified enzyme, 3 h, 37C, 50 microM FeSO4, 100 mg/ml catalase, 160 microM oxoglutarate, 1 mM ascorbate, 60 microM substrate (S- or R-naringenin) in 10 mM Tris/HCl buffer, pH 8.0
apigenin + succinate + CO2 + H2O
apigenin is converted by falvonoid 3',5'-hydroxylase to luteolin and tricetin
-
?
Organism
Organism
UniProt
Commentary
Textmining
Oryza sativa
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
expression of flavone synthase in Escherichia coli, purification on glutathione S-transferase (GST) affinity column
Oryza sativa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Oryza sativa
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
S-naringenin + 2-oxoglutarate + O2
purified enzyme, 3 h, 37C, 50 microM FeSO4, 100 mg/ml catalase, 160 microM oxoglutarate, 1 mM ascorbate, 60 microM substrate (S- or R-naringenin) in 10 mM Tris/HCl buffer, pH 8.0
699792
Oryza sativa
apigenin + succinate + CO2 + H2O
apigenin is converted by falvonoid 3',5'-hydroxylase to luteolin and tricetin
-
-
?
Synonyms
Synonyms
Commentary
Organism
flavone synthase
-
Oryza sativa
FNS
-
Oryza sativa
OsFNS
-
Oryza sativa
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
oxoglutarate
no flavone synthase activity without this compound
Oryza sativa
Cloned(Commentary) (protein specific)
Commentary
Organism
RT-PCR cloning of flavone synthase cDNA, subcloning in pGEX 5X-2 for expression in Escherichia coli
Oryza sativa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
S-naringenin
37C, 50 microM FeSO4, 100 mg/ml catalase, 160 microM oxoglutarate, 1 mM ascorbate, 60 microM substrate in 10 mM Tris/HCl buffer, pH 8.0
Oryza sativa
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
FeSO4
65% reduced activity without FeSO4
Oryza sativa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38700
-
SDS-PAGE, 1027 bp, Fe2+-binding site, oxoglutarate binding site
Oryza sativa
64700
-
SDS-PAGE, flavone synthase + glutathione S-transferase (26000 Da)
Oryza sativa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
S-naringenin + 2-oxoglutarate + O2
Oryza sativa
purified enzyme, 3 h, 37C, 50 microM FeSO4, 100 mg/ml catalase, 160 microM oxoglutarate, 1 mM ascorbate, 60 microM substrate (S- or R-naringenin) in 10 mM Tris/HCl buffer, pH 8.0
apigenin + succinate + CO2 + H2O
apigenin is converted by falvonoid 3',5'-hydroxylase to luteolin and tricetin
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
expression of flavone synthase in Escherichia coli, purification on glutathione S-transferase (GST) affinity column
Oryza sativa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Oryza sativa
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
S-naringenin + 2-oxoglutarate + O2
purified enzyme, 3 h, 37C, 50 microM FeSO4, 100 mg/ml catalase, 160 microM oxoglutarate, 1 mM ascorbate, 60 microM substrate (S- or R-naringenin) in 10 mM Tris/HCl buffer, pH 8.0
699792
Oryza sativa
apigenin + succinate + CO2 + H2O
apigenin is converted by falvonoid 3',5'-hydroxylase to luteolin and tricetin
-
-
?
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742512
Han
Functional characterization o ...
Plagiochasma appendiculatum
FEBS Lett.
588
2307-2314
2014
-
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1
-
13
-
-
2
-
-
-
-
-
4
-
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1
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3
1
2
1
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2
1
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1
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1
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13
-
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2
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-
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1
-
-
-
-
3
1
1
-
-
2
1
-
-
1
-
1
1
-
2
2
726253
Deguchi
Endogenous post-transcriptiona ...
Dahlia pinnata
Planta
237
1325-1335
2013
-
-
1
-
1
-
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-
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1
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1
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1
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1
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1
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1
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-
-
-
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-
1
1
-
-
-
713237
Fliegmann
Flavone synthase II (CYP93B16) ...
no activity in Glycine max
Phytochemistry
71
508-514
2010
-
-
-
-
-
-
-
-
-
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4
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-
-
-
-
699499
Jeon
Biological synthesis of 7-O-me ...
Populus deltoides
J. Microbiol. Biotechnol.
19
491-494
2009
-
1
1
-
-
-
-
-
-
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1
1
-
3
-
-
-
-
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1
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2
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1
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1
1
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1
1
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-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
685694
Yun
Expression of parsley flavone ...
Petroselinum crispum
Biosci. Biotechnol. Biochem.
72
968-973
2008
-
-
1
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
1
-
-
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-
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-
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1
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1
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-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685800
Lee
Characterization of flavone sy ...
Oryza sativa
BMB Rep.
41
68-71
2008
-
-
1
-
-
-
-
-
-
1
1
-
-
5
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
1
-
-
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-
-
1
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
699792
Jeong
-
Analysis of flavonoids and cha ...
Oryza sativa
J. Plant Biol.
51
97-101
2008
1
-
1
-
-
-
-
1
-
1
2
1
-
1
-
-
1
-
-
1
-
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1
-
3
-
-
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1
-
1
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1
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1
2
1
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1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
679173
Katsuyama
Synthesis of unnatural flavono ...
Petroselinum crispum
Chem. Biol.
14
613-621
2007
-
1
1
-
1
-
-
-
-
-
-
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-
3
-
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1
1
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1
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-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
689577
Gebhardt
Evolution of flavone synthase ...
Petroselinum crispum
Plant Physiol.
144
1442-1454
2007
-
-
1
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
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-
-
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-
1
-
-
1
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-
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-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
689585
Zhang
Flavone synthases from Medicag ...
Medicago truncatula
Plant Physiol.
144
741-751
2007
-
1
1
-
-
-
-
-
-
-
-
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1
-
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-
-
4
-
-
2
-
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-
-
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-
-
1
1
-
-
-
-
-
-
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-
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-
-
-
-
-
-
4
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671519
Leonard
Expression of a soluble flavon ...
Petroselinum crispum
Appl. Microbiol. Biotechnol.
70
85-91
2006
-
-
1
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
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-
1
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-
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-
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-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671524
Miyahisa
Combinatorial biosynthesis of ...
Petroselinum crispum
Appl. Microbiol. Biotechnol.
71
53-58
2006
-
-
1
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
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2
-
2
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-
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1
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-
-
2
-
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-
-
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671436
Leonard
Investigation of two distinct ...
Petroselinum crispum
Appl. Environ. Microbiol.
71
8241-8248
2005
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
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3
-
2
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-
-
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-
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-
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-
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1
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1
-
-
3
-
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-
-
-
-
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-
-
-
-
-
-
-
-
676365
Martens
Flavones and flavone synthases ...
Petroselinum crispum
Phytochemistry
66
2399-2407
2005
-
-
-
-
-
-
-
-
-
1
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-
4
-
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1
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2
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1
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1
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656956
Schroeder
Flavonoid methylation: a novel ...
Petroselinum crispum
Phytochemistry
65
1085-1094
2004
-
-
-
-
-
-
-
-
-
-
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2
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6
-
1
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6
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655572
Martens
Divergent evolution of flavono ...
Petroselinum crispum
FEBS Lett.
544
93-98
2003
-
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1
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4
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1
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2
-
2
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1
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1
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2
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654335
Lukacin
Purification and antigenicity ...
Petroselinum crispum
Arch. Biochem. Biophys.
393
177-183
2001
-
-
-
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6
-
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1
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1
1
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1
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1
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1
1
-
1
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-
-
-
-
-
-
656942
Martens
Cloning of parsley flavone syn ...
Petroselinum crispum
Phytochemistry
58
43-46
2001
-
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1
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3
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1
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1
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1
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1
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1
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-
439113
Britsch
Purification and characterizat ...
Petroselinum crispum
Arch. Biochem. Biophys.
282
152-160
1990
1
-
-
-
-
-
4
3
1
1
1
-
-
2
-
-
1
-
-
1
1
-
3
1
3
-
-
-
-
1
-
-
-
2
1
-
1
-
-
-
-
-
-
-
4
2
3
1
1
1
-
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-
-
1
-
1
1
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3
1
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1
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1
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