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Literature summary for 1.14.20.5 extracted from

  • Jeong, H.; Young, M.; Kim, B.; Ahn, J.
    Analysis of flavonoids and characterization of the OsFNS gene involved in flavone biosynthesis in rice (2008), J. Plant Biol., 51, 97-101.
No PubMed abstract available
Search for more literature for 1.14.20.5

Activating Compound

Activating Compound Comment Organism Structure
oxoglutarate no flavone synthase activity without this compound Oryza sativa

Cloned(Commentary)

Cloned (Comment) Organism
RT-PCR cloning of flavone synthase cDNA, subcloning in pGEX 5X-2 for expression in Escherichia coli Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 S-naringenin 37°C, 50 microM FeSO4, 100 mg/ml catalase, 160 microM oxoglutarate, 1 mM ascorbate, 60 microM substrate in 10 mM Tris/HCl buffer, pH 8.0 Oryza sativa

Metals/Ions

Metals/Ions Comment Organism Structure
FeSO4 65% reduced activity without FeSO4 Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38700
-
 SDS-PAGE, 1027 bp, Fe2+-binding site, oxoglutarate binding site Oryza sativa
64700
-
 SDS-PAGE, flavone synthase + glutathione S-transferase (26000 Da) Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-naringenin + 2-oxoglutarate + O2 Oryza sativa purified enzyme, 3 h, 37°C, 50 microM FeSO4, 100 mg/ml catalase, 160 microM oxoglutarate, 1 mM ascorbate, 60 microM substrate (S- or R-naringenin) in 10 mM Tris/HCl buffer, pH 8.0 apigenin + succinate + CO2 + H2O apigenin is converted by falvonoid 3',5'-hydroxylase to luteolin and tricetin ?

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
expression of flavone synthase in Escherichia coli, purification on glutathione S-transferase (GST) affinity column Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Oryza sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-naringenin + 2-oxoglutarate + O2 purified enzyme, 3 h, 37°C, 50 microM FeSO4, 100 mg/ml catalase, 160 microM oxoglutarate, 1 mM ascorbate, 60 microM substrate (S- or R-naringenin) in 10 mM Tris/HCl buffer, pH 8.0 Oryza sativa apigenin + succinate + CO2 + H2O apigenin is converted by falvonoid 3',5'-hydroxylase to luteolin and tricetin ?

Synonyms

Synonyms Comment Organism
flavone synthase
-
 Oryza sativa
FNS
-
 Oryza sativa
OsFNS
-
 Oryza sativa