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Literature summary for 1.14.20.5 extracted from
- Purification and characterization of flavone synthase I, a 2-oxoglutarate-dependent desaturase (1990), Arch. Biochem. Biophys., 282, 152-160.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | required for full activity, can not be replaced as a reductant by 2-mercaptoethanol or dithiothreitol | Petroselinum crispum |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,4-pyridinedicarboxylate | potent competitive | Petroselinum crispum | |
| Cu2+ | 0.02 mM, complete inhibition in presence of 0.01 mM Fe2+ | Petroselinum crispum | |
| prunin | competitive with respect to (2S)-naringenin | Petroselinum crispum | |
| Zn2+ | 0.02 mM, complete inhibition in presence of 0.01 mM Fe2+ | Petroselinum crispum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
2S-naringenin | - |
Petroselinum crispum | |
| 0.008 | - |
2S-eriodictyol | - |
Petroselinum crispum | |
| 0.016 | - |
2-oxoglutarate | in presence of 0.02 mM 2S-naringenin | Petroselinum crispum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Petroselinum crispum | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | required for full activity, cannot be replaced by Mn2+, Co2+ or Ni2+ | Petroselinum crispum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
gel filtration | Petroselinum crispum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Petroselinum crispum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Petroselinum crispum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Petroselinum crispum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0057 | - |
- |
Petroselinum crispum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2S)-eriodictyol + 2-oxoglutarate + O2 | - |
Petroselinum crispum | ? + CO2 + H2O | - |
? | |
| (2S)-naringenin + 2-oxoglutarate + O2 | - |
Petroselinum crispum | apigenin + succinate + H2O + CO2 | - |
? | |
| 2-hydroxynaringenin + 2-oxoglutarate + O2 | - |
Petroselinum crispum | ? + CO2 + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 24000-25000, SDS-PAGE | Petroselinum crispum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| flavone synthase I | - |
Petroselinum crispum |
| FNS I | - |
Petroselinum crispum |
| FS I | - |
Petroselinum crispum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 8.6 | - |
Petroselinum crispum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0018 | - |
Pyridine 2,4-dicarboxylate | - |
Petroselinum crispum | |
| 0.057 | - |
prunin | - |
Petroselinum crispum | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Petroselinum crispum | isoelectric focusing, pH gradient 6.6-4.0 | - |
4.9 |
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