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Literature summary for 1.14.20.4 extracted from

  • Xu, F.; Cheng, H.; Cai, R.; Li, L.L.; Chang, J.; Zhu, J.; Zhang, F.X.; Chen, L.J.; Wang, Y.; Cheng, S.H.; Cheng, S.Y.
    Molecular cloning and function analysis of an anthocyanidin synthase gene from Ginkgo biloba, and its expression in abiotic stress responses (2008), Mol. Cells, 26, 536-547.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Ginkgo biloba

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
with a His-tag, Western Blot, SDS-PAGE Ginkgo biloba

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Ginkgo biloba catalyzes the penultimate step in the biosynthesis of the anthocyanin class of flavonoids, from the colorless leucoanthocyanidins to the colored anthocyanidins ?
-
?
additional information Ginkgo biloba expression in abiotic stress responses. Up-regulated by all of the six tested abiotic stresses, UV-B, abscisic acid, sucrose, salicylic acid, cold and ethylene, consistent with the promoter region analysis of GbANS ?
-
?

Organism

Organism UniProt Comment Textmining
Ginkgo biloba B2M0X8 1 year old seedlings and 12 year old grafts
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography Ginkgo biloba

Source Tissue

Source Tissue Comment Organism Textmining
fruit young and mature. Weakly expressed in young fruits, highest level of GbANS mRNA is observed in mature fruits Ginkgo biloba
-
leaf young and mature. Weakly expressed in mature leaves Ginkgo biloba
-
additional information GbANS transcripts are not detected in the stems and roots Ginkgo biloba
-
plant ovule moderate expression level Ginkgo biloba
-
seedling and graft Ginkgo biloba
-
stamen moderate expression level Ginkgo biloba
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-cis-leucocyanidin + 2-oxoglutarate + O2
-
Ginkgo biloba cyanidin + succinate + CO2
-
?
dihydroquercetin + 2-oxoglutarate + O2
-
Ginkgo biloba quercetin + succinate + CO2
-
?
leucocyanidin + 2-oxoglutarate + O2 + H+
-
Ginkgo biloba cyanidin + succinate + CO2 + 2 H2O
-
?
additional information catalyzes the penultimate step in the biosynthesis of the anthocyanin class of flavonoids, from the colorless leucoanthocyanidins to the colored anthocyanidins Ginkgo biloba ?
-
?
additional information expression in abiotic stress responses. Up-regulated by all of the six tested abiotic stresses, UV-B, abscisic acid, sucrose, salicylic acid, cold and ethylene, consistent with the promoter region analysis of GbANS Ginkgo biloba ?
-
?

Synonyms

Synonyms Comment Organism
ANS
-
Ginkgo biloba
anthocyanidin synthase
-
Ginkgo biloba
GbANS bifunctional enzyme within the anthocyanidin and flavonol biosynthetic pathway Ginkgo biloba
leucoanthocyanidin oxygenase 2-oxoglutarate iron-dependent oxygenase Ginkgo biloba

pI Value

Organism Comment pI Value Maximum pI Value
Ginkgo biloba
-
-
5.52