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Literature summary for 1.14.20.15 extracted from

  • Vaillancourt, F.H.; Yin, J.; Walsh, C.T.
    SyrB2 in syringomycin E biosynthesis is a nonheme FeII alpha-ketoglutarate- and O2-dependent halogenase (2005), Proc. Natl. Acad. Sci. USA, 102, 10111-10116 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as His-tagged fusion protein Pseudomonas syringae pv. syringae

General Stability

General Stability Organism
autooxidative destruction after a small number of turnovers, the enzyme catalyze 7 turnovers before inactivation Pseudomonas syringae pv. syringae

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the reaction requires Fe2+ Pseudomonas syringae pv. syringae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-threonyl-[L-threonyl-carrier protein SyrB1] + 2-oxoglutarate + O2 + Cl- Pseudomonas syringae pv. syringae the enzyme participates in syringomycin E biosynthesis 4-chloro-L-threonyl-[L-threonyl-carrier protein SyrB1] + succinate + CO2 + H2O
-
?
L-threonyl-[L-threonyl-carrier protein SyrB1] + 2-oxoglutarate + O2 + Cl- Pseudomonas syringae pv. syringae B301D the enzyme participates in syringomycin E biosynthesis 4-chloro-L-threonyl-[L-threonyl-carrier protein SyrB1] + succinate + CO2 + H2O
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas syringae pv. syringae Q9RBY6
-
-
Pseudomonas syringae pv. syringae B301D Q9RBY6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas syringae pv. syringae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-threonyl-[L-threonyl-carrier protein SyrB1] + 2-oxoglutarate + O2 + Cl- the enzyme participates in syringomycin E biosynthesis Pseudomonas syringae pv. syringae 4-chloro-L-threonyl-[L-threonyl-carrier protein SyrB1] + succinate + CO2 + H2O
-
?
L-threonyl-[L-threonyl-carrier protein SyrB1] + 2-oxoglutarate + O2 + Cl- the enzyme can not chlorinate free L-Thr or the small molecule surrogate for L-Thr-S-SyrB1, L-Thr-S-N-acetylcysteamine Pseudomonas syringae pv. syringae 4-chloro-L-threonyl-[L-threonyl-carrier protein SyrB1] + succinate + CO2 + H2O
-
?
L-threonyl-[L-threonyl-carrier protein SyrB1] + 2-oxoglutarate + O2 + Cl- the enzyme participates in syringomycin E biosynthesis Pseudomonas syringae pv. syringae B301D 4-chloro-L-threonyl-[L-threonyl-carrier protein SyrB1] + succinate + CO2 + H2O
-
?
L-threonyl-[L-threonyl-carrier protein SyrB1] + 2-oxoglutarate + O2 + Cl- the enzyme can not chlorinate free L-Thr or the small molecule surrogate for L-Thr-S-SyrB1, L-Thr-S-N-acetylcysteamine Pseudomonas syringae pv. syringae B301D 4-chloro-L-threonyl-[L-threonyl-carrier protein SyrB1] + succinate + CO2 + H2O
-
?

Synonyms

Synonyms Comment Organism
SyrB2
-
Pseudomonas syringae pv. syringae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Pseudomonas syringae pv. syringae

General Information

General Information Comment Organism
metabolism the enzyme participates in syringomycin E biosynthesis Pseudomonas syringae pv. syringae