Protein Variants | Comment | Organism |
---|---|---|
A118D | suppressed H-bonding interaction of Arg254 with Cl-Fe(III)-OH and suppressed chlorination activity | Pseudomonas syringae pv. syringae |
A118Q | suppressed H-bonding interaction of Arg254 with Cl-Fe(III)-OH and suppressed chlorination activity | Pseudomonas syringae pv. syringae |
R247A | suppressed chlorination activity | Pseudomonas syringae pv. syringae |
R247Q | suppressed chlorination activity | Pseudomonas syringae pv. syringae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | Fe2+-dependent enzyme | Pseudomonas syringae pv. syringae | |
Iron | the enzyme employs an Fe(IV)-oxo species to achieve selective C-H halogenation of L-threonine. Combined quantum mechanical/molecular mechanical (QM/MM) calculations show the presence of three Cl-Fe(IV)-oxo isomers which interconvert. The one having its oxo ligand pointing toward the target C-H bond is active during the hydrogen atom abstraction (H-abstraction) process. The fate of the formed Cl-FeIII-OH/R radical intermediate is determined by a hydrogenbonding interaction between the Arg254 residue and the OH ligand of Cl-Fe(III)-OH | Pseudomonas syringae pv. syringae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas syringae pv. syringae | Q9RBY6 | - |
- |
Pseudomonas syringae pv. syringae B301D | Q9RBY6 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
SyrB2 | - |
Pseudomonas syringae pv. syringae |
syringomycin biosynthesis enzyme 2 | - |
Pseudomonas syringae pv. syringae |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the syringomycin E biosynthetic pathway | Pseudomonas syringae pv. syringae |