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Literature summary for 1.14.20.15 extracted from

  • Huang, J.; Li, C.; Wang, B.; Sharon, D.; Wu, W.; Shaik, S.
    Selective chlorination of substrates by the halogenase SyrB2 is controlled by the protein according to a combined quantum mechanics/molecular mechanics and molecular dynamics study (2016), ACS Catal., 6, 2694-2704 .
No PubMed abstract available

Protein Variants

Protein Variants Comment Organism
A118D suppressed H-bonding interaction of Arg254 with Cl-Fe(III)-OH and suppressed chlorination activity Pseudomonas syringae pv. syringae
A118Q suppressed H-bonding interaction of Arg254 with Cl-Fe(III)-OH and suppressed chlorination activity Pseudomonas syringae pv. syringae
R247A suppressed chlorination activity Pseudomonas syringae pv. syringae
R247Q suppressed chlorination activity Pseudomonas syringae pv. syringae

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ Fe2+-dependent enzyme Pseudomonas syringae pv. syringae
Iron the enzyme employs an Fe(IV)-oxo species to achieve selective C-H halogenation of L-threonine. Combined quantum mechanical/molecular mechanical (QM/MM) calculations show the presence of three Cl-Fe(IV)-oxo isomers which interconvert. The one having its oxo ligand pointing toward the target C-H bond is active during the hydrogen atom abstraction (H-abstraction) process. The fate of the formed Cl-FeIII-OH/R radical intermediate is determined by a hydrogenbonding interaction between the Arg254 residue and the OH ligand of Cl-Fe(III)-OH Pseudomonas syringae pv. syringae

Organism

Organism UniProt Comment Textmining
Pseudomonas syringae pv. syringae Q9RBY6
-
-
Pseudomonas syringae pv. syringae B301D Q9RBY6
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-

Synonyms

Synonyms Comment Organism
SyrB2
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Pseudomonas syringae pv. syringae
syringomycin biosynthesis enzyme 2
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Pseudomonas syringae pv. syringae

General Information

General Information Comment Organism
metabolism the enzyme is involved in the syringomycin E biosynthetic pathway Pseudomonas syringae pv. syringae