Literature summary for 1.14.20.12 extracted from

Brady, S.; Clardy, J.
Cloning and heterologous expression of isocyanide biosynthetic genes from environmental DNA (2005), Angew. Chem. Int. Ed. Engl., 44, 7063-7065 .

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Organism

Organism	UniProt	Comment	Textmining
uncultured organism	Q108L2	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2S)-3-(1H-indol-3-yl)-2-isocyanopropanoate + 2-oxoglutarate + O2	-	uncultured organism	3-[(E)-2-isocyanoethenyl]-1H-indole + succinate + 2 CO2 + H2O	-	?

Synonyms

Synonyms	Comment	Organism
isnB	-	uncultured organism

General Information

General Information	Comment	Organism
physiological function	presence of isonitrile synthase IsnA and IsnB is necessary and sufficient to generate 3-[(E)-2-isocyanoethenyl]-1H-indole from L-Trp and ribulose 5-phosphate. The isocyanide group in (2S)-3-(1H-indol-3-yl)-2-isocyanopropanoate is formed in a single enzymatic step on IsnA and the isocyanide-containing intermediate is oxidatively decarboxylated by IsnB to give 3-[(E)-2-isocyanoethenyl]-1H-indole. Both the nitrogen atoms in 3-[(E)-2-isocyanoethenyl]-1H-indole, including the isocyanide nitrogen atom, are derived from tryptophan	uncultured organism

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Release 2023.1 (January 2023)