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Literature summary for 1.14.20.1 extracted from

  • An, Y.; Dong, H.; Liu, G.
    Expression of cefF significantly decreased deacetoxycephalosporin C formation during cephalosporin C production in Acremonium chrysogenum (2012), J. Ind. Microbiol. Biotechnol., 39, 269-274.
    View publication on PubMed
Search for more literature for 1.14.20.1

Cloned(Commentary)

Cloned (Comment) Organism
gene cefEF Acremonium chrysogenum

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required Acremonium chrysogenum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
penicillin N + 2-oxoglutarate + O2 Acremonium chrysogenum
-
 deacetoxycephalosporin C + succinate + CO2 + H2O
-
 ?
penicillin N + 2-oxoglutarate + O2 Acremonium chrysogenum CGMCC3.3795
-
 deacetoxycephalosporin C + succinate + CO2 + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Acremonium chrysogenum P11935 formerly Cephalosporium acremonium, gene cefEF
-
Acremonium chrysogenum CGMCC3.3795 P11935 formerly Cephalosporium acremonium, gene cefEF
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
penicillin N + 2-oxoglutarate + O2
-
 Acremonium chrysogenum deacetoxycephalosporin C + succinate + CO2 + H2O
-
 ?
penicillin N + 2-oxoglutarate + O2
-
 Acremonium chrysogenum CGMCC3.3795 deacetoxycephalosporin C + succinate + CO2 + H2O
-
 ?

Synonyms

Synonyms Comment Organism
Cephalosporin biosynthesis expandase/hydroxylase UniProt Acremonium chrysogenum
DAOC/DAC synthase
-
 Acremonium chrysogenum

General Information

General Information Comment Organism
metabolism the enzyme from Acremonium chrysogenum is bifunctional and catalyzes both the synthesis of deacetoxycephalosporin C from penicillin N, EC 1.14.20.1, as well as the hydroxylation of deacetoxycephalosporin C to deacetylcephalosporin C, EC 1.14.11.26. The activities are located on two different domains Acremonium chrysogenum
additional information additional recombinant expression of gene cefF from Streptomyces clavuligerus in Acremonium chrysogenum strain CGMCC3.3795 leads to a reduction of the content of deacetoxycephalosporin C in the cephalosporin C fermentation broth, quantitative PCR expression analysis, overview Acremonium chrysogenum