Protein Variants | Comment | Organism |
---|---|---|
R308A | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Acremonium chrysogenum |
R308C | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Acremonium chrysogenum |
R308D | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Acremonium chrysogenum |
R308E | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Acremonium chrysogenum |
R308F | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Acremonium chrysogenum |
R308G | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Acremonium chrysogenum |
R308H | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Acremonium chrysogenum |
R308I | site-directed mutagenesis, the mutant shows significant improvement in the ability to convert penicillin analogues compared to the wild-type enzyme. The mutant shows the highest reactivity for penicillin G, with 3fold increase in kcat/Km ratio and 7fold increase in relative activity | Acremonium chrysogenum |
R308K | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Acremonium chrysogenum |
R308L | site-directed mutagenesis, the mutant shows significant improvement in the ability to convert penicillin analogues compared to the wild-type enzyme | Acremonium chrysogenum |
R308M | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Acremonium chrysogenum |
R308N | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Acremonium chrysogenum |
R308P | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Acremonium chrysogenum |
R308Q | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Acremonium chrysogenum |
R308S | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Acremonium chrysogenum |
R308T | site-directed mutagenesis, the mutant shows significant improvement in the ability to convert penicillin analogues compared to the wild-type enzyme | Acremonium chrysogenum |
R308V | site-directed mutagenesis, the mutant shows significant improvement in the ability to convert penicillin analogues compared to the wild-type enzyme | Acremonium chrysogenum |
R308W | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Acremonium chrysogenum |
R308Y | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme | Acremonium chrysogenum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
penicillin G | wild-type enzyme, pH not specified in the publication, temperature not specified in the publication | Acremonium chrysogenum | |
8.2 | - |
penicillin G | mutant R308I, pH not specified in the publication, temperature not specified in the publication | Acremonium chrysogenum | |
11 | - |
penicillin G | mutant R308L, pH not specified in the publication, temperature not specified in the publication | Acremonium chrysogenum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deacetoxycephalosporin C + 2-oxoglutarate + O2 | Acremonium chrysogenum | - |
deacetylcephalosporin C + succinate + CO2 + H2O | - |
? | |
additional information | Acremonium chrysogenum | a bifunctional enzyme that catalyzes both the ring-expansion of penicillin N to deacetoxycephalosporin C and the hydroxylation of the latter to deacetylcephalosporin C | ? | - |
? | |
penicillin N + 2-oxoglutarate + O2 | Acremonium chrysogenum | - |
deacetoxycephalosporin C + succinate + CO2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acremonium chrysogenum | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
relative activities of wild-type and R308 mutants with penicillin variants and analogue substrates, overview | Acremonium chrysogenum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-aminopenicillanic acid + 2-oxoglutarate + O2 | - |
Acremonium chrysogenum | ? + succinate + CO2 + H2O | - |
? | |
amoxicillin + 2-oxoglutarate + O2 | - |
Acremonium chrysogenum | ? + succinate + CO2 + H2O | - |
? | |
ampicillin + 2-oxoglutarate + O2 | - |
Acremonium chrysogenum | ? + succinate + CO2 + H2O | - |
? | |
carbenicillin + 2-oxoglutarate + O2 | - |
Acremonium chrysogenum | ? + succinate + CO2 + H2O | - |
? | |
deacetoxycephalosporin C + 2-oxoglutarate + O2 | - |
Acremonium chrysogenum | deacetylcephalosporin C + succinate + CO2 + H2O | - |
? | |
additional information | a bifunctional enzyme that catalyzes both the ring-expansion of penicillin N to deacetoxycephalosporin C and the hydroxylation of the latter to deacetylcephalosporin C | Acremonium chrysogenum | ? | - |
? | |
additional information | role of residue R308 in controlling substrate selectivity, substrate specificity of wild-type and mutant enzymes, overview | Acremonium chrysogenum | ? | - |
? | |
penicillin G + 2-oxoglutarate + O2 | - |
Acremonium chrysogenum | phenylacetyl-7-aminodeacetoxycephalosporanic acid + succinate + CO2 + H2O | - |
? | |
penicillin N + 2-oxoglutarate + O2 | - |
Acremonium chrysogenum | deacetoxycephalosporin C + succinate + CO2 + H2O | - |
? | |
penicillin V + 2-oxoglutarate + O2 | - |
Acremonium chrysogenum | ? + succinate + CO2 + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acDAOC/DACS | - |
Acremonium chrysogenum |
deacetoxy/deacetylcephalosporin C synthase | - |
Acremonium chrysogenum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0057 | - |
penicillin G | wild-type enzyme, pH not specified in the publication, temperature not specified in the publication | Acremonium chrysogenum | |
0.048 | - |
penicillin G | mutant R308L, pH not specified in the publication, temperature not specified in the publication | Acremonium chrysogenum | |
0.069 | - |
penicillin G | mutant R308I, pH not specified in the publication, temperature not specified in the publication | Acremonium chrysogenum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
penicillin G | wild-type enzyme, pH not specified in the publication, temperature not specified in the publication | Acremonium chrysogenum | |
0.0045 | - |
penicillin G | mutant R308L, pH not specified in the publication, temperature not specified in the publication | Acremonium chrysogenum | |
0.0085 | - |
penicillin G | mutant R308I, pH not specified in the publication, temperature not specified in the publication | Acremonium chrysogenum |