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Literature summary for 1.14.20.1 extracted from

  • Dotzlaf, J.E.; Yeh, W.K.
    Copurification and characterization of deacetoxycephalosporin C synthetase/hydroxylase from Cephalosporium acremonium (1987), J. Bacteriol., 169, 1611-1618.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.20.1

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis-2-nitrobenzoic acid 1 mM, 100% inhibition of expandase reaction, EC 1.14.20.1 Acremonium chrysogenum
Ca2+ weak Acremonium chrysogenum
EDTA 0.6 mM, 89% inhibition of expandase reaction, EC 1.14.20.1 Acremonium chrysogenum
iodoacetic acid 1 mM, 4% inhibition of expandase reaction, EC 1.14.20.1 Acremonium chrysogenum
N-ethylmaleimide 1 mM, 51% inhibition of expandase reaction, EC 1.14.20.1 Acremonium chrysogenum
o-phenanthroline 0.6 mM, 100% inhibition of expandase reaction, EC 1.14.20.1 Acremonium chrysogenum
p-hydroxymercuribenzoate 1 mM, 100% inhibition of expandase reaction, EC 1.14.20.1 Acremonium chrysogenum
Zn2+ strong Acremonium chrysogenum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
 2-oxoglutarate pH 7.5, 30°C Acremonium chrysogenum
0.029
-
 Penicillin N pH 7.5, 30°C Acremonium chrysogenum

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required for both activities Acremonium chrysogenum
additional information Fe2+ cannot be replaced by Mg2+, Mn2+, Ca2+, Co2+, Cu2+, Ni2+ or Zn2+ Acremonium chrysogenum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 1 * 41000, SDS-PAGE Acremonium chrysogenum
43000
-
 gel filtration Acremonium chrysogenum

Organism

Organism UniProt Comment Textmining
Acremonium chrysogenum
-
 catalyzes both reaction of EC 1.14.20.1 and of EC 1.14.11.26
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.827
-
 pH 7.5, 30°C Acremonium chrysogenum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
penicillin N + 2-oxoglutarate + O2
-
 Acremonium chrysogenum deacetoxycephalosporin C + succinate + CO2 + H2O
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 41000, SDS-PAGE Acremonium chrysogenum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
26 34 expandase reaction, EC 1.14.20.1 Acremonium chrysogenum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 7.8 expandase reaction, EC 1.14.20.1 Acremonium chrysogenum

pI Value

Organism Comment pI Value Maximum pI Value
Acremonium chrysogenum isoelectric focusing
-
 6.3