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Literature summary for 1.14.19.9 extracted from
- Enzymatic halogenation of tryptophan on a gram scale (2015), Angew. Chem. Int. Ed. Engl., 54, 298-301 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression in Escherichia coli | Lentzea aerocolonigenes |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | for optimization of the enzymatic regioselective halogenation process, a method that is easily scalable up to gram scale is established. An active FAD-dependent halogenase is co-immobilized with all auxiliary enzymes necessary for halogenation, formation of cross-linked enzyme aggregates (CLEAs) might stabilize its quaternary structure | Lentzea aerocolonigenes |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lentzea aerocolonigenes | Q8KHZ8 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-hydroxy-L-tryptophan + FADH2 + chloride + O2 + H+ | regioselective halogenation, recombinant enzyme | Lentzea aerocolonigenes | 7-chloro-5-hydroxy-L-tryptophan + FAD + 2 H2O | - |
? |  |
| D-tryptophan + FADH2 + bromide + O2 + H+ | regioselective halogenation, recombinant enzyme | Lentzea aerocolonigenes | 7-bromo-D-tryptophan + FAD + 2 H2O | - |
? | |
| L-tryptophan + FADH2 + bromide + O2 + H+ | best substrates, regioselective halogenation, recombinant enzyme | Lentzea aerocolonigenes | 7-bromo-L-tryptophan + FAD + 2 H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Lentzea aerocolonigenes |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| flavin-dependent tryptophan halogenase | - |
Lentzea aerocolonigenes |
| RebH | - |
Lentzea aerocolonigenes |
| Trp-7-halogenase | - |
Lentzea aerocolonigenes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Lentzea aerocolonigenes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Lentzea aerocolonigenes |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | FAD-dependent halogenase | Lentzea aerocolonigenes | |
| NAD+ | - |
Lentzea aerocolonigenes | |
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Release 2023.1 (January 2023)
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