Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Pseudomonas fluorescens |
Protein Variants | Comment | Organism |
---|---|---|
F103A | the mutant shows increased Km and reduced kcat values compared to the wild type enzyme | Pseudomonas fluorescens |
F103A/W455A | inactive | Pseudomonas fluorescens |
H101A | the mutant shows increased Km and reduced kcat values compared to the wild type enzyme | Pseudomonas fluorescens |
W455A | the mutant shows increased Km and reduced kcat values compared to the wild type enzyme | Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0499 | - |
tryptophan | His-tagged recombinant wild type enzyme, at 30°C, pH not specified in the publication | Pseudomonas fluorescens | |
0.1986 | - |
tryptophan | His-tagged recombinant mutant enzyme F103A, at 30°C, pH not specified in the publication | Pseudomonas fluorescens | |
1.785 | - |
tryptophan | His-tagged recombinant mutant enzyme H101A, at 30°C, pH not specified in the publication | Pseudomonas fluorescens | |
1.814 | - |
tryptophan | His-tagged recombinant mutant enzyme W455A, at 30°C, pH not specified in the publication | Pseudomonas fluorescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tryptophan + FADH2 + Cl- + O2 + H+ | Pseudomonas fluorescens | - |
7-chloro-L-tryptophan + FAD + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel chelating Sepharose column chromatography | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tryptophan + FADH2 + Cl- + O2 + H+ | - |
Pseudomonas fluorescens | 7-chloro-L-tryptophan + FAD + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PrnA | - |
Pseudomonas fluorescens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
tryptophan | His-tagged recombinant mutant enzyme W455A, at 30°C, pH not specified in the publication | Pseudomonas fluorescens | |
0.021 | - |
tryptophan | His-tagged recombinant mutant enzyme H101A, at 30°C, pH not specified in the publication | Pseudomonas fluorescens | |
0.067 | - |
tryptophan | His-tagged recombinant mutant enzyme F103A, at 30°C, pH not specified in the publication | Pseudomonas fluorescens | |
0.11 | - |
tryptophan | His-tagged recombinant wild type enzyme, at 30°C, pH not specified in the publication | Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | dependent on | Pseudomonas fluorescens |