Protein Variants | Comment | Organism |
---|---|---|
additional information | gene disruption mutant, no production of pyrroindomycin B, but still production of pyrroindomycin A | Lentzea aerocolonigenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
L-tryptophan | pH 7.5 | Lentzea aerocolonigenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lentzea aerocolonigenes | Q8KHZ8 | Halogenation requires the presence of RebF, which catalyzes the NADH-dependent reduction of FAD to provide FADH2 for the halogenase | - |
Lentzea aerocolonigenes 39243 | Q8KHZ8 | Halogenation requires the presence of RebF, which catalyzes the NADH-dependent reduction of FAD to provide FADH2 for the halogenase | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Lentzea aerocolonigenes |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
tryptophan + FADH2 + chloride + O2 + H+ = 7-chloro-L-tryptophan + FAD + 2 H2O | proposed mechanism: after formation of a FAD-OOH intermediate, the reactive chlorine is generated as a FAD-O-Cl intermediate. Chlorination of L-tryptophan proceeds by attack of the aromatic pi electrons on the intermediate in a two-electron mechanism and abstraction of a proton generates the final product | Lentzea aerocolonigenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + FADH2 + Br- + O2 + H+ | - |
Lentzea aerocolonigenes | 7-bromo-L-tryptophan + FAD + H2O | identity of product is confirmed by ESI-MS and 1H-NMR. Reaction is selective for the 7-position. Brominating activity is about 75% of chlorinating activity | ? | |
L-tryptophan + FADH2 + Br- + O2 + H+ | - |
Lentzea aerocolonigenes 39243 | 7-bromo-L-tryptophan + FAD + H2O | identity of product is confirmed by ESI-MS and 1H-NMR. Reaction is selective for the 7-position. Brominating activity is about 75% of chlorinating activity | ? | |
L-tryptophan + FADH2 + Cl- + O2 + H+ | - |
Lentzea aerocolonigenes | 7-chloro-L-tryptophan + FAD + H2O | identity of product is confirmed by ESI-MS and 1H-NMR | ? | |
L-tryptophan + FADH2 + Cl- + O2 + H+ | - |
Lentzea aerocolonigenes 39243 | 7-chloro-L-tryptophan + FAD + H2O | identity of product is confirmed by ESI-MS and 1H-NMR | ? | |
additional information | O2 and presence of RebF, which catalyzes the NADH-dependent reduction of FAD to provide FADH2 for the halogenase, are required for reaction. 54% of available L-tryptophan are converted within 5 min when the reaction is prepapred anaerobically and O2 is introduced slowly. Reaction rate drops by 90% in air-saturated conditions. No substrate: fluoride, iodide | Lentzea aerocolonigenes | ? | - |
? | |
additional information | O2 and presence of RebF, which catalyzes the NADH-dependent reduction of FAD to provide FADH2 for the halogenase, are required for reaction. 54% of available L-tryptophan are converted within 5 min when the reaction is prepapred anaerobically and O2 is introduced slowly. Reaction rate drops by 90% in air-saturated conditions. No substrate: fluoride, iodide | Lentzea aerocolonigenes 39243 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RebH | - |
Lentzea aerocolonigenes |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
L-tryptophan | pH 7.5 | Lentzea aerocolonigenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FADH2 | - |
Lentzea aerocolonigenes | |
additional information | no cofactor: NADPH, FMN | Lentzea aerocolonigenes |